模拟常温物流下大菱鲆菌相变化规律与优势腐败菌鉴定

Changes of bacterial flora composition and identification of the dominant spoilage bacteria in turbot under simulated of room temperature logistics

以大菱鲆为研究对象,将传统选择性培养基法和16S r DNA序列分析法相结合,并通过系统发育树分析,探索模拟常温物流下大菱鲆的菌相变化规律及优势腐败菌。结果表明:模拟常温物流下,传统分析与16S r DNA序列分析均表明,大菱鲆初始菌相与贮藏末期菌相明显不同。经16S r DNA序列、NCBI数据库比对以及系统发育树的构建表明,大菱鲆末期菌相主要有气单胞菌(Ameromonas sp.)、肠杆菌(Enterobacteriaceae sp.)、红杆菌(Rhodobacteraceae sp.)、发光杆菌(Photo bacterium sp.)、变形杆菌(Gammaproteobacteria sp.)、希瓦氏菌(Shewanella sp.)、拟杆菌(Bacteroidales sp.)和腈基降解菌(Nitriliruptoraceae sp.),其中气单胞菌(Aeromonas sp.)和肠杆菌中的柠檬酸杆菌(Citrobacter sp.)和哈夫尼氏菌(Hafnia sp.)为大菱鲆模拟常温物流下的优势腐败菌。

Turbots stored under simulated of room temperature logistics were studied in this essay. Selective medium method and 16S rDNA sequence analysis technology were used to study the change of bacterial flora composition in turbot during simulated of room temperature logistics. The results showed that the micro flora composition of turbot were different before and after storage.The 16S rDNA analysis, NCBI database and phylogenetic tree showed that the micro flora composition were Ameromonas sp. , Enterobacteriaceae sp., Rhodobacteraceae sp., Photo bacterium sp., Gammaproteobacteria sp., Shewanella sp., Bacteroidales sp.and Nitriliruptoraceae sp..The dominant spoilage bacteria of turbot stored in simulated of room temperature logistics were Aeromonas sp., Citrobacter sp.and Hafnia sp.