锌和土霉素胁迫对玉米种子发芽和幼苗抗氧化酶活性的影响

Zinc and oxytetracycline stress effects on maize germination and seedling antioxidant system

为了研究抗生素和重金属复合胁迫对玉米的毒性机制及环境健康风险,本研究以玉米(Zea mays L.)为材料,用低浓度锌(Zn)和土霉素(OTC)溶液培养玉米种子发芽,另外将健康玉米幼苗置于加入低浓度锌和土霉素的营养液中以水培的方式培养,研究Zn与OTC复合胁迫对玉米种子发芽和幼苗抗氧化系统的影响。结果表明,OTC和Zn单一胁迫时,对玉米种子发芽率影响不显著。而OTC和Zn复合处理能够显著促进玉米种子发芽率。各浓度OTC和Zn单一胁迫处理对玉米幼苗抗氧化酶活性的影响均为先升高后下降,Zn的加入可以减轻OTC对玉米幼苗的毒害作用。

In order to study the toxicity mechanism and environmental health risks about antibiotics and heavy metals compound stress on corn,maize（ Zea mays L.） seedlings were used as materials,and the method of hydroponic cultivation with low concentration of zinc（ Zn） and oxytetracycline（ OTC） was applied to treat maize seed. Effect of compound pollution between Zn and OTC on maize germination and seedling antioxidant system was studied. The results showed that the single stress of OTC and Zn had no significant influence on the seed germination rate of maize,but their combined stress could significantly improve the germination rate of maize seeds. The antioxidant enzyme activities of maize seedlings increased first and then decreased under all concentrations of OTC and Zn,and the addition of Zn could reduce the toxic effects of OTC on maize seedlings.