桃磷酸肌醇特异性磷脂酶C基因家族鉴定与分析

Genome-wide analysis and identification of phosphoinositide-specific phospholipase C gene family in Lovell peach( Prunus persica L.)

为了对桃(Prunus persica var.Lovell)中PI-PLC家族基因进行系统分析,利用桃基因组数据库,通过生物信息学手段,鉴定桃PI-PLC家族基因的基因结构、染色体定位和编码蛋白,通过序列比对进行进化和分类分析。结果表明,桃中含有5个PI-PLC家族基因,预测他们均含有9个外显子,分布于桃的2条染色体上。保守结构域分析结果显示,桃中PI-PLC蛋白均含有4个保守的EF、X、Y和C2结构域。进化树分析结果表明桃PI-PLC家族基因可分为4个亚家族。

In order to analyze PI-PLC gene family in Lovell peach（ Prunus persica L.）,bioinformatics method was used based on the peach genome database to obtain peach PI-PLC family genes structure and their positions on chromosome and duplication information. PI-PLC proteins were classified according to their phylogenetic relationship. The results showed that five PI-PLC genes existed in the peach（ P. persica L.） genome and located on two chromosomes. The results of conserved domains indicated that all peach PI-PLC proteins contained four conserved domains（ EF,X,Y and C2）. Phylogenetic analysis revealed that the peach PI-PLC gene family could be divided into four groups.