卒中后抑郁大鼠血清同型半胱氨酸与叶酸水平的表达变化

Expression changes of Hcy and FA levels in rats models with post-stroke depression

目的观察卒中后抑郁(PSD)大鼠血清中同型半胱氨酸(Homocysteine,Hcy)与叶酸(Folic acid,FA)水平的表达变化。方法将48只雄性Sprague-Dawley(SD)大鼠随机分成正常组、抑郁组、卒中组、PSD组,每组12只。卒中组应用线栓法制成局灶性大脑中动脉闭塞模型;抑郁组应用慢性不可测的温和刺激结合孤养法制成抑郁模型;PSD组先应用线栓法制备局灶性大脑中动脉闭塞模型,后结合慢性不可测的温和刺激及孤养法制成PSD模型。ELISA法检测血清中Hcy与FA水平。结果 PSD组与其他各组相比较,血清中Hcy水平明显升高,而血清中FA水平则明显降低,差异有统计学意义(P<0.01);抑郁组及卒中组分别与正常组相比较,血清中Hcy水平均明显升高,而FA水平明显降低,差异有统计学意义(P<0.01);卒中组血清中FA水平明显低于抑郁组,差异有统计学意义(P<0.05);而抑郁组与卒中组血清中Hcy水平相比较,差异无统计学意义(P>0.05)。结论卒中后抑郁大鼠模型的血清Hcy水平升高以及FA水平降低,有可能与卒中后抑郁的发病机制相关。

Objective To explore expression changes of Homocysteine( Hcy) and Folic acid( FA) levels in rats with post-stroke depression( PSD).Methods 48 adult male Sprague-Dawley( SD) rats were randomly divided into normal group,depression group,stroke group and PSD group,with 12 cases in each group. Focal cerebral ischemia models established by intraluminal suture method were used in the stroke group. In the depression group,depression models were made by chronic unpredictable mild stimulation combined with separate breeding. In the PSD group,focal cerebral ischemia models established by intraluminal suture method were used at first,and then PSD rat models established by chronic unpredictable mild stimulation combined with separate breeding were applied. ELISA was used to detect the levels of Hcy and FA in serum. Results Compared with the other 3 groups,serum Hcy levels in the PSD group were significantly higher,but FA levels were significantly lower,difference was statistically significant( P< 0.01). Compared with the normal group,serum Hcy levels in the depression group and the stroke group were significantly higher,but FA levels of both groups were significantly lower,difference was statistically significant( P<0. 01). The serum FA levels of the depression group was significantly higher than that of the stroke group,difference was statistically significant( P<0.05),whereas there was no statistically significant difference between serum Hcy levels of the depression group and the stroke group( P>0.05).Conclusion Increase of serum Hcy levels and decrease of serum FA levels of PSD rats models may be related with the pathogenesis of PSD.