β-磷酸三钙预构中心血管化人工骨的实验研究

An experimental study on the central vascularized artificial bone with beta tricalcium phosphate

目的:观察β-磷酸三钙构建中心血管化人工骨的效果,探索人工骨血管化机制及血管植入的可行性。方法:将兔的腰背动脉解剖分离形成血管束,实验组将血管束植入人工骨侧槽内,并用自体微小颗粒骨填满,埋入背阔肌肌袋内,对侧不植入血管作为对照。术后4、8、12周行螺旋CT、X线和组织学检查。结果:实验组人工骨形成类似滋养孔的结构,有大量新生血管自中央向四周生长,4周时开始出现新生骨,12周新生骨更趋于成熟。对照组有血管自周围长入,新生骨少且不成熟。术后4周,实验组和对照组CT值、X线及组织学评分比较,差异无统计学意义;术后8、12周,实验组和对照组CT值、X线及组织学评分比较差异有统计学意义(P<0.05)。结论:血管植入人工骨可显著促进人工骨血管化,该方法可作为构建血管化骨模型。

Objective： To investigate the effects of construction of the central vascularized artificial bone with β-tricalcium phosphote and explore the mechanism of vascularization in artificial bone for its further clinical application. Methods： We built a model by implanting the selected New Zealand rabbits lumbar dorsal artery side channel of the β-tricalcium phosphate, filled with the autologous tiny bone particles, and flushed the latissimus dorsi muscle bag. Pure artificial bone was put into every rabbit＇s lift latissimus dorsi muscle bag as the control group. The samples were taken out for spiral CT, X-ray, and histology after 4, 8, and 12 weeks. Results： The site where the implanted vascular bundles appeared like foramen nutriens, the blood vessels were abundant, which emerged from deep tissue of the artificial bone were evident on the surface specimen, the central vascularized tricalcium phosphte were filled with new-born vessels at 4-8 weeks. Newly formed bone appeared after 4 weeks and was more mature at 12 weeks. There were only a few new-born vessels in control. Four weeks postoperatively, there were no significant differences on CT, X-ray, and histological grading between the experimental group and the control group （P〉0.05）; 8, 12 weeks postoperatively, the differ ence was significant on CT, X-ray, and histological grading between the experimental group and the control group （P〈0.05）. Conclusion： Putting an artery into central axis of the artificial bone and implanting the artificial bone into rabbit＇ s latissimus dorsi muscle can notably improve vascularization of artificial bone. This method can be used to build model of vascularized bone.