葡萄糖分子印迹电极测定唾液中葡萄糖

Glucose Molecularly Imprinted Electrode Detects Glucose in Saliva

采用分子印迹技术、邻苯二胺为功能单体,通过循环伏安法对该电极进行性能考察。结果表明,该分子印迹电极对葡萄糖具有较好的灵敏度与选择性,线性范围为1×10^(-9)~1×10^(-8) mol/L,检出限(S/N=3)为1×10^(-9) mol/L。将电极用于实际样品的分析,方法回收率为96.3%~100.9%,RSD为0.15%~4.4%。测定了多个口腔唾液样本中葡萄糖的含量,检测结果均在8.37~12.68 mg/L范围内。由此认为,该电极可以直接用于检测人体口腔唾液葡萄糖含量,实现对人体中血糖水平无创性检测。

Molecularly imprinted membrane （OPOD） was prepared by molecular imprinting technique to synthesize poly（o）-benzene two amine molecules on the surface of glassy carbon electrode （GCE） by using the two amines as functional monomers. K3 [Fe（CN）6] was used as probe molecule, and the electrode was investigated by cyclic voltammetry. The results showed that the imprinted electrode for Glu with good sensitivity and selectivity, the linear range for 1×10^-9 -1×10^-8 mol/L, the detection limit （S/N=3） was 1×10^-9mol/L, the electrode has good reproducibility and stability. The electrode was used for the analysis of the actual saliva samples. The recovery rate was between 96.3% and 100.9%. Determination of the content of a plurality of different normal human oral salivary Glu detection, and the result is great, all fall within the normal range of detection that the electrode can be used for direct detection of saliva Glu detection, realize ofnon-invasive detection whether excessive blood sugar.