北京地区黄檗分布与环境因子的关系

Relationship between the distribution of Phellodendron amurense and environmental factors in the Beijing area

黄檗(Phellodendron amurense Rupr.)为我国二级重点保护野生植物,在北京地区黄檗多散生于阔叶林中,数量稀少。为了解北京地区黄檗分布与环境因子的关系,促进种群扩繁,在北京百花山、松山和雾灵山自然保护区共设置了12个20 m×20 m的样地,利用CCA分析方法对不同地点黄檗的生长分布状况与海拔、坡度、坡向、郁闭度、土壤p H值、碱解氮和土壤有机质等11个环境因子的关系进行了分析。结果显示,CCA排序第一轴主要反映了海拔、郁闭度和坡度的变化,第二轴主要反映了有机质含量、碱解氮含量、p H值和坡向的变化,其中海拔、碱解氮和土壤有机质是影响黄檗生长分布的重要环境因子,低海拔、低碱解氮含量以及土壤有机质高的地段适宜黄檗分布。对影响黄檗分布的环境因子进行定量分离,结果发现环境因子对黄檗样地物种分布的解释能力为84.5%,显示出较好的排序效果,黄檗分布点受人为干扰较少,其所在植物群落与环境保持了良好的对应关系;环境因子与物种分布呈显著相关(P=0.03),表明CCA排序结果可以解释环境因子对物种分布的影响程度。

The CYP83B1 gene of Isatis indigotica Fort. was cloned and its expression patterns were analyzed. Results showed that the length of the Ii CYP83B1 gene was 1652 bp,and included two exons and one intron. The full length cDNA of IiCYP83B1 was 1500 bp,encoding a protein of 499 amino acids. IiCYP83B1 was a hydrophobic protein located in the endoplasmic reticulum,without a transmembrane domain or signal peptide. Its secondary structure mainly included alpha helixes and irregular coils. Homologous comparison illustrated that IiCYP83B1 has close relationship with Raphanus sativus Linn.,Brassica napus L.,Brassica oleracea L.,and Brassica rapa L. qRT- PCR analysis indicated that IiCYP83B1 was expressed in root,stem,flower,and fruit,and highly expressed in leaf. It was also highly expressed in the seedling,vegetative growth,and flowering stages,compared with the germination period.Moreover,Ii CYP83B1 could be induced significantly by methyl jasmonate（ MeJA） and glucose（ Glu）,but repressed by low temperature（ 4℃） and salicylic acid（ SA）. Results in this experiment provide reference for further functional study on IiCYP83B1.