miR-124靶向调节STAT3基因对甲状腺乳头状癌细胞增殖和侵袭的影响

The effect of mi R-124 on the proliferation and invasion of thyroid papillary cancer cells by targeting STAT3

目的探讨miR-124及STAT3在甲状腺乳头状癌组织中的表达,以及抑制或过表达miR-124对甲状腺乳头状癌细胞增殖和侵袭的影响。方法 Real-time PCR法检测17例患者甲状腺乳头状癌组织及对应的癌旁组织中miR-124的表达,Western bolt法检测STAT3蛋白的表达。将miR-124 inhibitors及miR-124 mimics转染至甲状腺乳头状癌Kl细胞株,Western blot方法检测转染后STAT3蛋白的表达变化,CCK-8方法和克隆形成实验检测转染后Kl细胞的增殖和侵袭情况。结果 miR-124在甲状腺乳头状癌组织中的表达明显低于癌旁组织,STAT3蛋白在甲状腺乳头状癌组织中的表达明显高于癌旁组织(P<0.01)。转染miR-124 inhibitors的K1细胞增殖侵袭能力与对照组相比显著升高,细胞内STAT3蛋白表达亦明显升高(P<0.01);转染miR-124 mimics的K1细胞增殖侵袭能力与对照组相比显著降低,细胞内STAT3蛋白表达亦明显降低(P<0.01)。结论 miR-124抑制甲状腺乳头状癌Kl细胞的增殖和侵袭可能与下调STAT3的表达相关。

Objective To study the expressions of miR-124 and STAT3 in thyroid papillary caneer（PTC） tissue and the effect of inhibitors or mimics of miR-124 on the proliferation and invasion of thyroid papillary cancer K1 ceils. Methods The expression of miR-124 in 17 cases of thyroid papillary cancer tissues and corresponding para-carcinoma tissues was detected by real time polymerase chain reaction （RT-PCR）, and Western blot method was employed to detect the expression of STAT3 protein. The expression of STAT3 was detected by Western blot, proliferation and invasion ability of thyroid papillary cancer K1 cells were detected by CCK-8 method and clone formation experiments respectively after the miR-206 inhibitors or miR-206 mimics was transfected. Results The expression level of miR-124 in thyroid papillary cancer tissue was significantly lower than that in para-carcinoma normal tissues（P〈0.01）, but was significantly higher for STAT3 protein in thyroid papillary cancer than in para-earcinoma tissue（P〈0.01）. The ability of cell proliferation and invasion of K1 ceils as well as the expression level of STAT3 protein was increased significantly after miR-124 inhibitors was transfected, but decreased significantly after miR-124 mimics was transfected（P〈0.01）. Conclusion The inhibition of MiR-124 on the proliferation and invasion abilities of K 1 cells might be related to down-regulating the expression of STAT3.