miR-23a/b在非小细胞肺癌的表达及临床意义

Expression and clinical significance of miR-23a/b in non small cell lung cancer

目的研究miR-23a和miR-23b在非小细胞肺癌中的表达特征,并探讨其临床意义。方法收集157例非小细胞肺癌手术切除标本及50例癌旁组织标本,采用Real time PCR方法检测miR-23a和miR-23b在非小细胞肺癌及其癌旁组织中的表达,分析二者表达的相关性,并探讨其表达与临床病理特征及其预后的关系。结果 miR-23a和miR-23b在非小细胞肺癌组织中的表达水平均高于癌旁肺组织,二者在非小细胞肺癌组织中表达呈正相关(r=0.351,P<0.001)。miR-23a和miR-23b联合高表达与淋巴结有无转移(P<0.001)、远处转移(P=0.001)及临床分期(P=0.002)相关,而与年龄、性别、组织类型及组织分化程度无显著相关性(P>0.05)。KaplanMeier分析显示miR-23a和miR-23b联合高表达组患者生存期显著低于单独高表达组或联合低表达组(P=0.009),Cox风险比例模型分析显示miR-23a和miR-23b联合高表达为非小细胞肺癌患者的危险因素。结论 miR-23a和miR-23b在非小细胞肺癌中异常高表达可能是潜在的肺癌预后分子标志物。

Objective To analyze the expression profile of miR-23a and miR-23b in non small cell lung cancer（NSCLC） and the clinical significance. Methods 157 cases surgical specimens of NSCLC patients and 50 cases adjacent normal tissue were collected, miR-23a and miR-23b expression were detected by real-time PCR, The correlation of miR-23a and miR-23b expression in NSCLC tissues, and with clinicopathological factors and prognosis were analyzed. Results The expression levels of miR-23a and miR-23b were higher in NSCLC than in adjacent normal lung tissue, and the expression of miR-23a and miR-23b was positively correlated （r=0.351, F〈0.001）,and positively correlated to lymph node metastasis（P〈0.001） and distant metastasis（P=0.001） and clinical staging（P--0.002）, but not to age, sex, histologic type and the degree of histological differentiation（P〉0.05）. The overall survival of patients was significantly lower in combined high expression miR-23a and miR-23b group than in single high expression or combined low expression group（P=0.009）, Cox proportional hazards model analysis showed that combined high expression of miR-23a and miR-23b was a risk factor for NSCLC patients. Conclusion The combined high expression of miR-23a and miR-23b is a potential marker for predicting the prognosis of NSCLC.