上海地区常用近交系小鼠品系的单核苷酸多态性分型研究

The Single Nucleotide Polymorphism Genotyping of Inbred Mice from Shanghai

目的比较上海地区常用近交系小鼠单核苷酸多态性(SNP)位点分型情况。方法采用多重PCR-LDR检测技术,选取上海地区2家单位的10个近交系小鼠,对21条染色体上的44个SNP位点进行分型检测,同时,随机选取上海地区2个近交系品系作为双盲样本,对分型方案进行验证,差异位点利用测序法进行验证。结果同一来源的10个近交系小鼠,同品系间SNP分型结果完全一致;不同种群来源6个品系SNP分型结果提示,BALB/c,FVB,DBA/2和C3H/He在44个位点上分型结果均相同,CBA和C57BL/6分别在7个和2个位点存在差异;差异位点经测序验证结果与SNP分型方案一致。结论上海地区常用近交系小鼠中,同一来源的品系中SNP遗传质量具有良好稳定性和一致性,不同种群来源的相同品系间SNP分型存在差异。

Objective To analyze the single nucleotide polymorphism （SNP） genotyping of inbred mice from Shanghai. Methods 44 SNP loci on 21 chromosomes of 10 inbred mice strains from 2 laboratory animal companies of Shanghai were detected by the PCR-LDR genotyping panels, and the other 2 inbred mice strains were selected as the double blind samples for verification. And discrepancy locus were verified by sequencing. Results The SNP genotyping results of all the samples from the same inbred mice strains were homomorphism, but 6 inbred mice strains from different populations （BALB/c, FVB, DBA/2 and C3H/He） were polymorphism with 7 and 2 discrepancy locus respectively. The sequencing results to verify the discrepancy locus were the same as the SNP genotyping results. Conclusions The SNP genotyping was very stable and consistent in inbred mice strains from the same population in Shanghai, and the SNP genotyping of the same inbred mice strains from different populations was polymorphism.