小鼠剪尾出血模型的优化

Improvement on Tail Tip Bleeding Model in Mice

喃要]目的探讨小鼠剪尾出血模型在凝血药物体内评价过程中应用的可行性。方法对传统鼠尾出血模型实验过程进行考察和比较分析,对小鼠剪尾模型制备方法进行优化,建立了一种可快速准确检测鼠尾出血量和出血时间的方法。并利用商品化凝血酶药物苏灵验证了该方法在凝血药物药效学评价中的有效性,对鼠尾出血量(出血时间)与小鼠凝血时间指标的敏感性比较也显示了此模型的优越性。结果优化后的鼠尾出血模型可对小鼠出血量进行低至微升级的精确检测,通过一定时间间隔的出血量来确定出血终点的方法,很大程度上消除了实验中的人为误差。该模型操作简便、易于使用,可以敏感且有效模拟体外创伤的动物模型,在评价凝血药物的药效中发挥作用。结论小鼠剪尾出血模型可以敏感反应凝血药物的体内作用,可作为凝血药物初步筛选和评价的有效工具。

Objective The application of tail tip bleeding model in mice was explored to evaluate the hemostatic activity of some coagulation components. Method This tail tip bleeding model improved to be used as a trauma model in the aspect of coagulant evaluation was compared with the traditional operation process. The new method was established for fast quantitative detection of the blood loss and decided the bleeding time from the starting point when the tail was cut off to the end point when the blood loss was less than 1 μL in two minutes intervals. Saculin approved as a coagulant drug on sale in domestic market was used to verify the effectiveness of this assay. The sensitivity between coagulate time and blood loss （or bleeding time） is also compared in mice. Results The advanced tail tip bleeding model in mice can quantitative analysis blood loss and bleeding time in order to reduce the personal error to a great extent. This model is easy to use and can simulate the process of trauma properly. Conclusion The tail tip bleeding model in mice can reflect the action of the coagulant drug sensitively, which is a useful tool for screening and assessment of coagulants.