外固定架制作兔股骨延迟愈合模型

Establishment of Femoral Delayed Healing Model with External Fixation in Rabbit

目的使用外固定架制作兔股骨骨折延迟愈合模型。方法将72只新西兰白兔随机分成对照组,实验A组、B组,将所有兔左侧股骨中段截骨,以微型可调节外固定架为固定装置,实验A组及B组去除10 mm皮质骨,同时去除内外骨膜及骨髓,实验A组保持骨折断端1 mm间隙,实验B组使用骨蜡封堵骨折断端,然后保持骨折断端接触。对照组截断骨皮质后即行外固定,不去除骨膜及骨髓。术后通过大体观察、影像学观察及组织学方法观察截骨端的愈合情况。结果实验A组及B组愈合时间均为12周,对照组愈合时间为4周。结论该实验建立的兔股骨骨折延迟愈合模型具有延迟愈合的X线及病理特征,说明该模型制作合理、可靠。

Objective To establish a model of rabbit femoral delayed healing with the extemal fixation. Methods Seventy and two New Zealand white rabbits were randomly divided into three groups, including the control group, the experimental group A and experimental group B. Make osteotomies of all the animals＇ left femur, is in the middle, and make the micro adjustable external fixator as the fixtures. 10mm cortical bone has been removed of the experimental group A and group B while removing the inner and outer periosteum and bone marrow. Keep the experimental group A lmm gap to the fracture site. The experimental group B has been using bone wax plugging the fracture site, while maintained contact with the fracture fragments. The control group cut off the cortical bone and then fixed with the external fixator, without removing the periosteum and bone marrow. Observe the healing of osteotomy by gross observation, radiographic and histological observation postoperatively. Results The healing time of the experimental group A and group B are 12 weeks, while the control group are four weeks. Conclu- sion The rabbit femur fracture delayed union model has X-ray and pathological features of delayed healing, indicating that the model is reasonable and reliable.