摘要
为了建立一种操作性强、稳定、高效的小鼠生精细胞体外培养体系,从10只8周龄成年雄性健康清洁级昆明小鼠睾丸组织分离出细胞得到混悬液,采用支持细胞和生精细胞共培养方式,通过添加多种细胞培养液,以维持细胞的生长和增殖。结果表明:采用睾丸生精细胞、支持细胞共同培养的方式可得到密度较大的生精细胞,且精原细胞、精母细胞的数量占绝大多数。
The present study was conducted to develop a simple,stable and efficient in vitro culture system for mouse germ cells.Testiculus organization originally from 10 Kunming mice at 8 weeks were got and adopt to suspension liquid,and co-cultured with Sertoli cells and germ cells together and adding the various cell culture solutions keeping cell growth and survive.The results showed that the co-culturing system of Sertoli cells and germ cells were preferable.
出处
《畜牧与兽医》
北大核心
2017年第2期15-17,共3页
Animal Husbandry & Veterinary Medicine
基金
2014年度新疆农业职业技术学院资助课题(XJNZYKJ2014004
XJNZYKJ2014003)
石河子大学动植物育种专项(gxjs2013-yz07)
关键词
小鼠
生精细胞
分离
体外培养
mouse
spermatogenic cell
separation
in vitro culture
