摘要
为了有效地控制绵羊的后代性别,选取3只健康和繁殖性能正常的成年雄性湖羊,1只湖羊作为对照组,另外2只湖羊分别采用质粒p LL3.7-A、p LL3.7-B对Zfy mRNA基因进行干扰,采集精液并利用人工授精与216只母羊进行配种,观察母羊及后代繁殖情况。结果显示:与对照组相比,干扰组湖羊的后代性别比例发生明显的偏移,雌性比例达64%以上,而子代的初生重、繁殖性能等均无变化。结论:干扰Zfy基因生产绵羊性控精液的方法简单、方便、易操作,在生产中有一定的推广价值和应用前景。
In order to control the offspring sex more effectively,the RNA interference technique of the Zfy gene was used and the results were analyzed by chi-square test in the healthy male Hu sheep.The results showed that the sex ratio of offspring in two experimental Hu sheep was significantly changed and the female proportion of the offspring after insemination with 216 female Hu Sheep was over 64%,while birth weights and reproductive performance of the offspring were not changed.Our results suggested that the RNA interference of the Zfy gene in sheep for sex control semen was simple,convenient and easy to operate.
出处
《畜牧与兽医》
北大核心
2017年第2期47-50,共4页
Animal Husbandry & Veterinary Medicine
基金
2014年度新疆农业职业技术学院资助课题(XJNZYKJ2014003)
兵团科技支疆项目(2014AB012)
