松属素和木犀草素抗肿瘤活性比较

Different biological activities of pinocembrin and luteolin

为比较松属素和木犀草素两种黄酮类化合物的抗肿瘤功效,将正常培养的肺腺癌细胞(A549)、乳腺癌细胞(MCF-7,MDA-MB-231)及神经胶质瘤细胞(U87、U1172)经不同浓度的松属素和木犀草素(40,80,160μm)分别处理24和48 h,倒置显微镜观察细胞形态,SRB法检测细胞存活率;吖啶橙染色观察细胞核凝集和片段化;Hoechst 33258检测细胞凋亡;Western blot检测膜联蛋白A7(ANXA7)、procaspase3和LC3B的表达。结果表明,松属素和木犀草素以剂量依赖的方式抑制A549、MCF-7和MDA-MB-231细胞增殖,而对U87和U172细胞的抑制功效较弱。Western blot结果表明,在MDA-MB-231细胞中,松属素和木犀草素上调LC3-Ⅱ的水平,促进细胞凋亡。这说明松属素和木犀草素对肺腺癌和乳腺癌细胞具有抗肿瘤功效。

To study the antitumor activities of pinocembrin and luteolin, the normal cultured human lung carcinoma （A549）, human breast cancer （MCF-7, MDA-MB-231） and glioma cells （U87,Ul172） were treated with different concentrations of pinocembrin and luteolin （40, 80, 160 μm） for 24 and 48 h, respectively. The cell viability was measured by SRB method. Hoechst 33258 staining and acridine orange staining were used to observe nuclear fragmentation, respectively. The levels of proeaspase3, ANXA7 and LC3B were tested by western blotting. Pinocembrin and luteolin significantly inhibited proliferation of A549, MCF-7 and MDA-MB-231 cells in a time- and dose dependent manner. However, the inhibitory effects on U87 and U172 was not significantly. Pinocembrin and luteolin also induced apoptosis and autophagy by upregulating the levels of caspase 3 Pinocembrin and luteolin are potential antitumor agents especially to human lung carcinoma and breast cancer cells.