北京市3221例门诊患者HPV 13个高危型别分型的实验研究

Experiment research of HPV 13 high-risk types in 3221 outpatients of Beijing

目的了解北京市门诊患者人乳头瘤病毒(human papilloma virus,HPV)13个高危型别的感染情况及在不同年龄、宫颈疾病中的分布情况。方法收集2012年4月到2014年2月门诊就诊患者的宫颈分泌物或泌尿生殖道分泌物标本,利用荧光定量PCR方法进行检测,用SPSS 16.0软件进行统计分析。结果在3 221例门诊患者中,HPV 13个高危型别的检出率为33.7%,其中男性患者650例,检出率为22.8%;女性患者2 571例,检出率为36.5%。年龄18~22岁、23~44岁和45~80岁组男性HPV 13个高危型别的检出率分别为14.7%、21.2%和37.9%,女性检出率分别为41.2%、33.4%和44.4%。结论北京市3221例门诊患者,HPV高危型总体阳性率较高的型别为HPV16型、HPV52型和HPV58型,女性感染HPV13个高危型别总阳性率高于男性;HPV高危型在男性与女性各个年龄组中的分布有差异,但HPV16型和HPV58型在两组中阳性率都较高;在男性门诊患者中,随着年龄的增加阳性率逐渐增加。对男性13个高危型别阳性者进行早期干预,在降低其女性伴侣宫颈疾病的发病率中具有重要意义。

Objective To study high-risk HPV typing and its distribution in 3 221 outpatients of Beijing. Methods Samples from the outpatient department from April 2012 to February 2014 were collected according to sample collection guide. Detectiion and typing were performed with fluorescence quantitative PCR method. Data were analyzed with statistically soft SPSS 16.0. Results Of all the 3 221 cases, the positive rate is 33.7%. 650 cases were male patients with the positive rate of 22.8%, 2 571 cases were female with the positive rate of 36.5%. According to age, all patients were divided into 3 groups. 18~22 years old, 23~44 years old and 45~80 years old. The positive rates of each group of male patients were 14.7%, 21.2% and 37.9%respectively, and the positive rates of female patients of each group were 41.2%, 33.4% and 44.4%respectively. Conclusion The main types of high-risk HPV in 3 221 outpatients of Beijing were HPV16,HPV52 and HPV58. The positive rate of female patients were higher than that of male patients. Although different groups of males and females have different distribution in typing, they are still in accordance with main types of HPV16 and HPV58. The infection rate of male patients increased along with the increasing age.Treatments with male positive patients have impartment significance for decrease cervical diseases.