MiR-214对人乳腺癌MCF-7细胞生物学行为的影响

Effect of miR-214 on biological function in breast cancer MCF-7 cells

目的:探讨微小RNA-214(miR-214)对人乳腺癌MCF-7细胞增殖、凋亡、迁移及周期分布等生物学行为的影响。方法:采用脂质体转染法将miR-214模拟物转入MCF-7细胞(实验组),以转入miR-214阴性对照的MCF-7细胞(阴性对照组)作对照。采用实时荧光定量PCR法检测miR-214的表达情况;CCK-8法检测细胞增殖能力的改变;流式细胞仪检测细胞凋亡及细胞周期;划痕愈合实验检测细胞的迁移。结果:实验组MCF-7细胞miR-214的表达较阴性对照组明显上调;实验组细胞的增殖能力明显受到抑制;实验组凋亡细胞所占比例明显高于阴性对照组(P=0.008),G1期细胞数增加,S期细胞数减少(P<0.05);划痕愈合实验表明,实验组细胞迁移能力明显受到抑制(P<0.001)。结论:MiR-214可能通过抑制乳腺癌MCF-7细胞增殖及迁移、促进细胞凋亡及影响细胞周期的分布而发挥抑癌作用。

Aim： To explore the effect of microRNA-214（miR-214） on the proliferation, apoptosis, migration and cell cycle distribution of human breast cancer MCF-7 cells. Methods： The miR-214 mimics were transfeeted into MCF-7 cells （experimental group） through liposome tranafection, and the MCF-7 ceils transfeeted by miR-214 negative control were used as control. The expression of miR-214 level was detected by real-time fluorogenic quantitative-PCR. The change of proliferation ability of MCF-7 cells was detected by CCK-8 method. The changes of apoptosis and cell cycle distribution were examined by flow cytometry. The change of migration ability was detected by wound healing assay. Results： After transfection with miR-214 mimics, the expression level of miR-214 in MCF-7 cells was up-regulated. Compared with con- trol, the cell proliferation of MCF-7 ceils in experimental group was inhibited, the cell apoptosis rate was elevated （P = 0.008） and the proportion of cells at Gj phase increased, and that of cells at S phase decreased（P 〈0.05） ,besides, the migration ability was inhibited（P 〈 0. 001 ）. Conclusion： MiR-214 may act as a tumor suppressor in MCF-7 cells through inhibiting cell proliferation and migration, promote cell apoptosis and affect cell cycle distribution.