同属不同种大黄土大黄苷含量测定

Determination of the Content of Emodin in the Same Kind of Loess

[目的]建立同属不同种大黄土大黄苷测定方法。[方法]色谱柱C18柱(Agilent TC-C18 analytical,4.6mm×250mm,5μm),流动相乙腈-水(25:75),柱温30.0℃,检测波长323nm,流速0.80mL·min^(-1)。用DAD检测器200~400nm对土大黄苷对照品和供试品色谱进行光谱扫描。[结果]药用大黄中未见土大黄苷色谱峰,河套大黄和华北大黄均可见土大黄苷色谱峰。提示正品大黄中无土大黄苷,伪品大黄中含有土大黄苷。土大黄苷对照品和供试品出现一致色谱峰,结果显示二峰的光谱扫描图一致。提示供试品含有土大黄苷。[结论]薄层色谱法和高效液相色谱法检测结果准确,可做为鉴别大黄药材的推荐方法。

[Objective] To establish a method for the determination of emodin in the same species. [Method] C 18 column （AgilentTC-C 18 analytical, 4.6mm × 250 mm, 5 μ m）, the mobile phase of acetonitrile water （25：75）, the column temperature was 30 degrees, the detection wavelength of 323nm, 0.80mL· min^-1. with DAD velocity detector 200-400nm of Rhaponticin control and the ehromatogram of the test spectra scanning. [Results] No soil rhubarb rhubarb aglycone peaks in large yellow and yellow in North are visible Rhaponticin peaks. Prompt soilless chrysophanin genuine rhubarb, rhubarb adulterants containing rhapontin. Rhapontin control products and samples the same chromatographic peaks, results show that the spectral scanning figure two consistent peak. Prompt samples containing rhapontin. [Conclusion] The results of TLC and HPLC are accurate and can be used as a method for the identification of rhubarb.