鸡血藤精准煮散饮片与原饮片质量的对比性研究

A Quality Comparison Between the Precise Powder Decoction Pieces and the Original Slices of Spatholobus suberectus Dunn.

目的:本研究采用化学指纹图谱法及DNA条形码分子鉴定技术对鸡血藤饮片煮散质量体系进行探索研究。方法:应用ITS2序列作为DNA条形码对鸡血藤进行鉴定,对比鸡血藤原饮片及精准煮散饮片的出膏率;收集3个批次鸡血藤饮片,采用HPLC法进行3批次饮片混合粉碎前后质量均一性、指纹图谱与相似度评价。结果:ITS2序列对鸡血藤药材可实现准确鉴定。鸡血藤煮散饮片出膏率及指标成分表儿茶素含量均略高于原饮片;原饮片批间表儿茶素溶出量有明显差异性RSD为11.0%,混合后制成煮散饮片后差异性明显降低RSD为1.0%。指纹图谱中14个共有峰相对峰面积均有所提高,RSD值明显减少。结论:鸡血藤精准煮散饮片提高原饮片的浸出率,成分溶出率及质量均一性,表明鸡血藤煮散饮片有较好的的临床应用优势。

In this study, DNA molecular identification technology and chemical fingerprint method were adopted to evaluate the quality system of precise powder decoction pieces（PPDP） of S. suberectus dunn（SSD）. ITS2 sequence was taken as DNA barcode to indentify SSD. Different specifications of PPDP were prepared, their dry extract contents were quantified in contrast with that of original slices. Three batches of SSD original slices were gleaned and the content uniformity, fingerprint and similarity evaluation before and after the mixing and pulverization were valued by HPLC-DAD. As a result, ITS2 successfully and accurately identified the SSD in this study. The extract rate of PPDP was 15.5%, 1.11 times as much as the original slices. RSD of inter-assay dissolution of cepicatechin from the original slices was 11.0%, which was reduced to 1.0% after mixing and preparing into PPDP. The relative peak area of the 14 common peaks identified by fingerpringts were larger, while the RSD values significantly decreased. It was concluded that the PPDP of SSD improved the extraction efficiency and uniformity of the original slices, featuring quite prospective in more reasonable and scientific clinical use.