大白菜抗TuMV分子标记辅助选择技术研究

Molecular Marker-Assisted Selection for Resistance to Turnip mosaic virus in Chinese Cabbage( Brassica campestris L. ssp. pekinensis)

分别以大白菜TuMV国家级抗源材料8407和高感TuMV的核心种质材料冠291为亲本构建分离群体,为验证TuMV抗性基因TuRBCS01两侧紧密连锁的分子标记m Br4055和Br ID10723的检测准确率,对上述两个亲本F_2代分离群体的107个单株进行自交构建F_(2∶3)家系,并对每个家系的TuMV抗性进行鉴定,以判断原F_2单株的TuMV抗性及基因型,同时利用上述两个标记引物对F_2代107个单株进行检测,根据检测结果及抗病性鉴定结果计算标记检测的准确率。结果表明,两标记均检测为纯合抗病的株系有22株,其中有2株经抗病性验证为杂合抗病,其余均为纯合抗病,检测准确率为90.9%;两标记均检测为杂合抗病的有48株,经抗病性验证,其中5株为纯合抗病,5株为纯合感病,其余均为杂合抗病,检测准确率为79.2%;两标记均检测为纯合感病的有23株,经抗病性验证,其中4株为杂合抗病,1株为纯合抗病,鉴定准确率为78.3%。上述检测结果为更好地利用标记mBr4055和BrID10723进行分子标记辅助选择奠定了基础。

The separation groups had been constructed using the national-level antigen material 8407 and the high susceptible and core germplasm material Guan 291 to Chinese cabbage TuMV as parents. In order to further verify the accuracy for detection of the molecular markers m Br4055 and Br ID10723,which located on the nearest both sides of TuMV resistance gene TuRBCS01,107 individuals of F2 generation were selfed to construct the F（2∶ 3）families. The TuMV resistance of each F（2∶3） line were identified in order to determine the TuMV resistance and gene type of original F2 individuals. The 107 individuals of F2 generation were amplified using the above two markers. The marker detection accuracy was calculated according to the results of amplification and TuMV resistance. The results showed that 22 individuals were amplified both homozygous resistant bands in the two marker loci. Among them,2 individuals were proved to be heterozygous genotype by disease resistance test. The others were homozygous resistance genotype. The detection accuracy was 90. 9%. And 48 individuals were amplified both heterozygous resistant bands in the two marker loci. Among them,5 individu-als were proved to be homozygous resistant genotype,5 individuals were homozygous susceptible genotype by disease resistance test. The others were heterozygous resistant genotype. The detection accuracy was 79. 2%.And 23 individuals were amplified both homozygous susceptible bands in the two marker loci. Among them,4individuals were proved to be heterozygous resistant genotype,one was homozygous resistant genotype by disease resistance test. The detection accuracy was 78. 3%. The above detection results laid a foundation for molecular marker assisted selection using markers m Br4055 and Br ID10723.