水稻稻瘟病菌单孢分离技术及常见问题分析

Single Spore Isolation Technology and Common Problem Analysis of Rice Blast Pathogen Magnaporthe girsea

水稻稻瘟病是由稻瘟菌(Magnaporthe girsea)引起的一种真菌性病害,可对水稻生产造成巨大损失。稻瘟菌的频繁变异易导致抗病品种抗性丧失,抗病育种家需每年对稻瘟菌变化情况进行监测。如何快速有效获得纯化菌株是监测稻瘟菌变化的前提。结合多年实践经验,本文详细介绍了稻瘟菌单孢分离技术以及分离过程中遇到的一些常见问题。这些研究结果可以指导育种家快速有效地获得纯化稻瘟菌。

Rice blast is a kind of epiphyte disease caused by Magnaporthe grisea,which can cause huge economic losses for rice production. The rice blast resistant cultivars easily lose the resistance in the field due to the frequent aberrance of pathogenicity of Magnaporthe grisea,so the rice breeders need to monitor the aberrance of pathogenicity of Magnaporthe grisea every year. How to quickly and efficiently obtain the purified Magnaporthe grisea strain is the precondition of monitoring the aberrance. Combined with practical experiences of many years,this study introduced the single spore isolation technology of Magnaporthe grisea in detail and analyzed some common problems encountered in the process of isolation. These results could instruct the rice breeders to obtain purified Magnaporthe grisea strain quickly and efficiently.