中医刮痧渗出物中免疫成分及含量的研究

Study on Immunological Components and Their Contents in Subcutaneous Exudate Induced by Cutaneous Scraping Method

【目的】观察大鼠刮痧后皮下渗出物中免疫因子成分和血液成分的变化,显微镜下比较刮痧前后皮肤组织病理学改变。【方法】将SD大鼠分成2大组,分别为刮痧组、未刮痧组。2大组再各分为3小组,分别进行血液及皮肤白细胞介素-1β(IL-1β)、IL-6、干扰素-γ(IFN-γ)含量检测,血常规检测以及皮肤病理学观察。【结果】刮痧组大鼠血液白细胞总数显著升高(P<0.05),皮肤IL-1β、IFN-γ含量显著升高(P<0.05),血液溶血率显著上升(P<0.05),但血液中IL-1β、IL-6、IFN-γ水平未见显著改变;显微镜下可见刮痧后皮肤严重水肿、血管充血扩张以及炎症细胞浸润。【结论】刮痧可快速调动机体免疫反应,"痧"中所含免疫成分有助于疾病的治疗。

Objective To investigate the changes of the immunological factors in subcutaneous exudate and blood components of the rats receiving cutaneous scraping method,and to compare the changes of skin histopathological features before and after cutaneous scraping under microscope. Methods SD rats were randomly divided into two groups,cutaneous-scraping group and non-cutaneous-scraping group. And then each group was divided into three subgroups. The observation indexes included the levels of interleukin（IL）-1β, IL-6 and interferon gamma（IFN-γ）in the blood and the skin,routine blood examination,and skin histopathological features. Results In cutaneous-scraping group,the number of white blood cells in the blood and the levels of IL-1β and IFN-γ in skin tissues were increased（P〈0.05）,the hemolysis rate was increased（P〈0.05）. However,the levels of IL-1β,IL-6 and IFN-γ in the blood showed no obvious changes. Under the microscope, severe skin edema, vascular congestion and dilatation, and infiltration of inflammatory cells were found in the skin after cutaneous scraping.Conclusion The cutaneous scraping method can activate the immune response rapidly, and the immunological components of the subcutaneous exudate after cutaneous scraping are helpful to the disease treatment.