神犀丹对内毒素休克大鼠多脏器损伤的保护作用

Protective Effect of Shenxi Dan on Multiple Organ Damage of Rats with Endotoxic Shock

【目的】观察凉血活血开窍方神犀丹对内毒素休克大鼠多脏器功能损伤的保护作用。【方法】将SD大鼠随机分为正常组、模型组和神犀丹组,除正常组外,其他2组大鼠腹腔注射D-氨基半乳糖(Gal N)和尾静脉注射脂多糖(LPS)复制内毒素休克模型,全程监测平均动脉压(MAP)的变化,分别在输注0、2、6 h时采集血清标本及肺肝组织,检测全血细胞、血清总胆红素(TB)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)、谷丙转氨酶(ALT)的含量,苏木素—伊红(HE)染色后光镜下观察大鼠肺肝组织的病理学改变。【结果】神犀丹组血压下降程度较模型组轻,血小板计数(PLT)无明显降低,血清TB、LDH、AST、ALT的含量上升幅度显著低于模型组,神犀丹组肺肝组织炎性病理改变较模型组明显减轻。【结论】神犀丹能改善内毒素休克大鼠的肺肝功能,减轻病理损害,改善微循环。

Objective To investigate the protective effect of Shenxi Dan, a recipe for cooling blood, activating blood and inducing resuscitation, on multiple organ damage of rats with endotoxic shock. Methods The SD rats were randomly divided into normal group,model group and Shenxi Dan group. Except for the normal group, the rats in the other groups were given intraperitoneal injection of D-galactosamine（Gal N） and intravenous injection of lipopolysaccharide（LPS） to induce endotoxic shock. The changes in mean arterial pressure（MAP）during the entire process were monitored. Serum samples and liver and lung tissues were collected after infusion for 0 h,2 h,6 h, respectively, and then the amount of whole blood cells, serum contents of total bilirubin（TB）, lactate dehydrogenase（LDH）,aspartate aminotransferase（AST）,and alanine aminotransferase（ALT） were detected.Meanwhile, the pathological changes in lung and liver tissues after hematoxylin-eosin（HE） staining were observed under light microscope. Results Compared with the model group, the decrease degree of the blood pressure was mild,platelet count（plt）was not decreased obviously,while the increase of serum TB,LDH,AST,ALT contents was significantly inferior,and inflammatory pathological changes of lung and liver tissues significantly were much relieved in Shenxi Dan group. Conclusion Shenxi Dan can improve lung and liver function of endotoxic shock rats,reduce the pathological damage,and improve microcirculation,which is helpful to clinical treatment of endotoxic shock.