和厚朴酚通过抑制氧化应激减轻脂多糖诱导的急性肺损伤

Honokiol Attenuates Lipopolysaccharide-induced Acute Lung Injury Through Inhibiting Oxidative Stress

【目的】探讨和厚朴酚对脂多糖(LPS)诱导的急性肺损伤(ALI)的影响及机制。【方法】选取40只SPF级BALB/c小鼠,随机分为5组:正常对照组,LPS组,和厚朴酚低、高剂量组,地塞米松组。应用LPS诱导小鼠ALI模型。和厚朴酚腹腔注射后,进行支气管肺泡灌洗液(BALF)中的中性粒细胞计数、白蛋白浓度、小鼠肺泡通透性、肺组织髓过氧化物酶(MPO)活性检测。采用试剂盒分析各组小鼠肺组织中丙二醛(MDA)、蛋白质羰基含量(PCC)、活性氧(ROS)、谷胱甘肽(CAT)、超氧化物歧化酶(SOD)活性、还原型谷胱甘肽过氧化氢酶(GPx)活性、谷胱甘肽-S-转移酶(GST)活性。【结果】LPS组小鼠BALF中的中性粒细胞计数、白蛋白浓度、MPO活性、伊文思蓝(EB)含量增加(P<0.05),抗氧化酶活性显著下降(P<0.05)。经和厚朴酚干预后,小鼠BALF中中性粒细胞计数、白蛋白浓度、MPO活性、EB含量及脂质过氧化水平显著下降,抗氧化酶活性显著升高(P<0.05)。【结论】和厚朴酚可通过抑制氧化应激减轻LPS诱导的ALI。

Objective To investigate of effect and mechanism of honokiol against acute lung injury（ALI）induced by lipopolysaccharide（LPS）. Methods Forty SPF BALB/c mice were randomly divided into 5 groups（N =8）, normal control group,LPS group,low-and high-dose magnolol groups,and dexamethasone group. The mouse model of ALI was induced by LPS. After intraperitoneal injection of honokiol, we detected neutrophil count,concentration of albumin,and pulmonary myeloperoxidase（MPO）activity in bronchoalveolar lavage fluid（BALF）as well as alveolar permeability. We also detected the levels of malondialdehyde（MDA）, protein carbonyl content（PCC）,reactive oxygen species（ROS）and glutathione（CAT）,and the activities of superoxide dismutase（SOD）,catalase,glutathione peroxidase（GPx）,and glutathione-S-transferase（GST）in lung tissue of mice.Results In the LPS group, the neutrophil count, albumin concentration, MPO activity and Evans blue（EB）content were increased（P〈0.05）,and anti-oxidase activity was decreased significantly（P〈0.05）. After treatment with honokiol, the neutrophil count, albumin concentration, MPO activity, EB content, and lipid peroxidation level were decreased significantly, and the activities of antioxidant enzymes were increased significantly（P〈0.05）. Conclusion Honokiol has protective effects against LPS-induced acute lung injury through inhibiting oxidative stress.