高分辨熔解曲线分析技术的发展与展望

The development and prospects of high-resolution melting analysis

高分辨熔解曲线分析技术简便快速、灵活性高、成本低、敏感性及特异性高、闭管操作的优势，使其成为临床实验诊断及科研工作中得到广泛应用的分子诊断技术之一。随着高分辨熔解曲线分析检测仪器、DNA染料及熔解曲线分析软件的发展，高分辨熔解曲线分析技术的敏感性、特异性及准确度不断提高，为遗传病、肿瘤、病原微生物及个体化用药等提供快速、高效、经济的分子诊断检测平台。（中华检验医学杂志，2017，40：77－79）

High-resolution melting analysis （ HRMA/HRM ）, a simple, rapid, flexible, inexpensive closed tube approach with high sensitivity and specificity has been one of the most widely used molecular diagnostic techniques in clinicalas well as in research settings .Recently, rapid development ofinstruments , DNA dyes and analysis software significantly enhance the sensitivity , specificity and accuracy of HRM,providing a fast, efficient and economic molecular diagnostic platform for molecular diagnosis of inherited disease , molecular profiling and target therapy of cancer , identification of pathogen , as well as individualized medicine.