摘要
目的通过观察活血化痰方对认知障碍大鼠认知功能及大脑皮层及海马区p53的影响,探讨其在改善大鼠认知障碍的机制。方法采用双侧颈总动脉阻断改良法制作大鼠慢性脑缺血模型,给予活血化痰方干预4周后,采用Morris水迷宫定位航行实验(place navigation)及空间探索实验(spatial probe)测定各组大鼠学习和记忆能力,western blot法检测乙酰化p53的含量变化情况。结果模型大鼠4周后显示逃避潜伏期增长,跨越平台次数减少,这种改变可以被活血化痰方所抑制。活血化痰方可以降低大鼠额叶皮层、海马、伏隔核处脑组织乙酰化p53的表达。结论活血化痰方可明显改善认知障碍大鼠学习记忆功能功能,其机制可能与该方抑制p53表达有关。
Objective To observe the effects of Huoxue huatan Formula cognitive fuction and acetylated p53 in congnitive impairment rats. Methods By bilateral common carotid artery occlusion to establish chronic cerebral ischemia model of rats, the learning and memory ability of the rats in each gruop were evaluated after the intervention of Huoxue Huatan Formula in 4 weeks by using Morris water maze test and Spatial probe test and detecting the contents of acetylated p53 changes by wesern blot. Results After 4 weeks of treatment, that escape latency period were found inceased while the times crossing the platform decreased which could inhibited by the decoction. The acetylated p53 expression were found decreased in frontal cortex, hippocampus and the nucleus accumbens of the rats. Conclusions HuoXue Huatan Formula could significantly improve cognitive function in congnitive impairment rats. The mechanism could be attributed to its inhibiton of Acetylated p53.
出处
《云南中医学院学报》
2017年第1期10-13,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
上海市普陀区中心医院院级课题(2013PT030)
