成纤维细胞激活蛋白α在神经胶质瘤中的表达及临床意义

Expression of fibroblast activation alpha protein in glioma and its significance

目的:探讨成纤维细胞激活蛋白α(FAP-α)在神经胶质瘤组织中的表达及其与临床病理特征和预后的关系。方法:应用免疫组织化学法检测33例神经胶质瘤和7例正常对照脑组织中FAP-α蛋白的表达,并结合临床病理资料进行分析。同时对所有病例进行随访,分析FAP-α表达与患者预后的关系。进一步检索脑肿瘤分子数据库(Rembrandt)探讨FAP m RNA表达与预后的关系。结果:低级别胶质瘤和高级别胶质瘤之间FAP-α蛋白的积分光密度值(IOD)差异有统计学意义(P<0.01),且与胶质瘤病理分级存在正相关(r=0.673,P<0.01)。7例正常脑组织FAP-α蛋白不表达或表达弱阳性,与低级别胶质瘤(P<0.05)和高级别胶质瘤(P<0.01)比较差异有统计学意义。FAP-α蛋白的表达强度与患者性别、肿瘤大小、年龄以及手术切除程度无明显相关性(P>0.05)。生存分析显示FAP-α低表达组和高表达组生存率差异有统计学意义(P<0.01),FAP-α的表达是影响胶质瘤患者预后的独立因素(P<0.05)。检索脑肿瘤分子数据库,发现FAP m RNA低表达预后明显好于高表达(P<0.01)。结论:FAP-α在神经胶质瘤中存在表达,且与神经胶质瘤的病理分级正相关,其高表达可能提示不良预后。

Objective： To explore the expression of FAP-α in human gliomas, and its relationship between clinical characteristics and prognosis. Methods： Expression level of FAP-ct protein was detected with immunohistochemistry in 33 cases of human gliomas and 7 cases of normal brain tissue, followed by subsequent clinicopathological correlative studies. Meanwhile, all patients were followed up, the relationship between the expression level of FAP-α and the glioma prognosis were studied. Furthermore, brain tumor molecular database （Rembrandt） was searched to investigate the relationship between the FAPmRNA expression and prognosis. Results： Comparing the expression intensities （IOD） of FAP-α protein in low-grade gliomas with those in high-grade gliomas, it was significantly different （P〈0.01）; the expressions of FAP-ct and the pathological grades of glioma were positively correlated （r=0.673, P〈0.01）. There was low or no expression in 7 cases of normal brain tissue; Comparing with low grade glioma （P〈0.05） and high grade glioma （P〈0.01）, there was significantly difference. The FAP-α expression was not significantly correlated with gender or tumor size or surgical degree and age （P〉0.05）. By survival analysis, overall survival time was significantly difference between the Low FAP-α and High FAP-α （P〈0.01）, and the expression of FAP-α was a independent prognostic factor in human gliomas （P〈0.05）. According to the results obtained from Rembrandt, patients with high FAP mRNA-expressing glioma showed statistically poorer prognoses compared with patients with low FAP mRNA-expressing glioma. Conclusion： FAP-α is expressed in human gliomas, which is positively correlated with the pathological grade of gliomas, and its high expression may suggest a poor prognosis.