循环microRNA-4281对肝癌的诊断与预后评估研究

Study of circulating microRNA-4281 for diagnosis and prognostic valuation in hepatocellular carcinoma

目的探讨循环miRNA-4281在肝癌患者血清中的表达情况及对诊断的临床意义。方法运用QRT-PCR技术分别检测45例肝癌患者(肝癌组)及正常人(对照组)血清中miRNA-4281的表达量。结果与对照组相比,肝癌患者血清标本中miRNA-4281的表达量明显高于正常人,且差异有统计学意义(P<0.05)。ROC曲线下面积是0.805,microRNA-4281作为肝癌诊断的灵敏度为84.40%,特异度是73.30%。miRNA-4281的表达与AFP无明显相关性。Kaplan-Meier分析提示miRNA-4281表达水平高的患者具有较短的生存期限(P<0.05)。结论检测miRNA-4281有利于肝癌的诊断,可作为HCC潜在的临床诊断标志物,同时其对于评估患者的预后具有重要作用。

Objective To measure the expression of the circulating miRNA-4281 in the serum of patients with hepatocellular carcinoma（ HCC）,and to find out the clinical meaning in diagnosis. Methods Blood samples from normal healthy（ n = 45） and HCC patients（ n = 45） were collected,respectively. Quantitative Real-time PCR was used to detect the expression level of miRNA-4281 in the two groups. Results The expression of miRNA-4281 was significantly higher in the patients compared to that in the control（ P0.05）.The area under receiver-operating characteristic curve was 0.805,with the sensitivities of 84.40% and the specificities of 73.30%.The expression level of miRNA-4281 and AFP had no correlation.Kaplan-Meier showed that the patients seemed to live a shorter time with the high expression of miRNA-4281（ P0. 05）. Conclusion Detecting miRNA-4281 may help to diagnose HCC.MiRNA-4281 may be a potential marker in the diagnosis and the prognosis.