NF-κB通路与川芎提取物抗脑缺血作用相关性研究

Protective Effects of Ligusticum Wallichii Extract on Cerebral Ischemia and the Correlation Study with NF-κB Pathway

目的研究川芎提取物抗脑缺血的作用及NF-κB机制。方法大脑中动脉栓塞法制备大鼠脑缺血再灌注模型,实验组给予川芎提取物(100mg/kg),每天灌胃给药1次,连续4周,模型组给予等量生理盐水,测定脑梗死面积,HE染色法检测脑组织的损伤程度。流式细胞仪检测氧糖剥夺胶质细胞的凋亡率,Pathway Finder Array法筛选出NF-κB信号通路被激活,免疫荧光法进一步检测NF-κB p65亚基是否入核。结果与模型组相比,川芎提取物能减少脑缺血再灌注大鼠的脑梗死面积,降低OGD胶质细胞的凋亡率,NF-κB信号通路在脑缺血再灌注后被激活,川芎提取物使pP65进入细胞核内的数量显著降低。结论川芎提取物对大鼠脑缺血损伤有保护作用,可能与抑制NF-κB信号通路的激活有关。

OBJECTIVE To study the protective effects of Ligusticum Wallichii extract on cerebral ischemia and the mecha nism. METHODS Male SD rats were divided into control group, model group and Ligusticum Wallichii extract （100 mg/kg） group. The rats in the treatment groups were administered once daily for 4 weeks. Middle cerebral artery occlusion method was used for cerebral ischemia-reperfusion model. After the model establishment, the neurological function scores were observed, the infarct size was measured by TTC staining, and the apoptosis rate of OGD glial cells by flow cytometry instrument, the ac- tivated NF-nB signaling pathway was measured Pathway Finder Array screening, the translocation of NF-κB p65 was measured by immunofluorescence. RESULTS Compared with the model group, Ligusticum Wallichii could significantly decrease the cer- ebral infarct size and the apoptosis rate of OGD glial cells Pathway Finder Array detection of NF-κB signaling pathways are ac- tivated in the normal saline group. Immunofluorescence test again important regulatory factors pP65 NF-κB signal is activated in the saline group into the nucleus. CONCLUSION The protection of extract from Ligusticum Wallichii on cerebral isehemia -reperfusion injury was related to NF-κB pathway.