竹节香附素A对人胃癌细胞株BGC-823细胞周期及STAT3信号通路的影响

The Effects of Raddeanin A on Human Gastric Cancer BGC-823 Cell Cycle and STAT3 Signaling Pathway

目的研究竹节香附素A(Raddeanin A,RA)在体外对胃癌细胞BGC-823的周期阻滞作用及对STAT3信号通路的影响。方法应用MTT法检测RA对人低分化细胞BGC-823细胞的增殖影响,应用流式细胞仪检测RA对BGC-823细胞周期的阻滞作用,细胞划痕实验检测RA对BGC-823迁移、侵袭能力的影响,应用Western blot检测其对STAT3信号通路的影响。结果 RA在给药12、24、48h后,与对照组相比,对BGC-823细胞的增殖抑制有显著效果,呈现时间-浓度依赖关系。流式细胞周期结果显示,RA作用于人胃癌细胞BGC-823 12h后,S期比例与对照组相较显著上升,而G2/M期比例下降,表明细胞被阻滞于S期。在浓度为8、16μmol/L时,镜下显示迁移过去的胃癌细胞数目均明显低于对照组,Western blot结果显示RA能下调p-STAT3蛋白的表达。结论 RA能有效抑制人胃癌细胞的增殖并阻滞其细胞周期于S期,并且抑制STAT3信号通路中p-STAT3的表达。

OBJECTIVE To investigate the effects of Raddeanin A（RA） on cell cycle and STAT3 signaling pathway in hu man gastric cancer cell line BGC-823. METHODS Effects of different concentrations of RA on proliferation of BGC-823 cell line were investigated with MTT assay; flow cytometry was used to detect the cell cycle inhibition; wounding heal assay was used to measure the influence of migration ability of RA on BGC-823 cell; the effect of STAT3 signaling pathway of RA on BGC-823 cell was measured by Western blot. RESULTS After the BGC-823 cells were treated by RA for 12, 24, 48 h, com- pared with control group, RA significantly inhibited the proliferation of BGC-823 cell （P〈0.01）. The inhibitive effects pres- ented a dose and time-dependent manner. Flow cytometry demonstrated that RA blocked the cell cycle in S phase; wounding heal assay revealed that RA inhibited the migration of BGC-823 cell. Western blot results showed that RA down-regulated the expression of phosphorylated STAT3 （p-STAT3）. CONCLUSION RA can effectively inhibit the proliferation of BGC-823 cell, block its cell cycle in S phase and inhibit the expression of p-STAT3.