摘要
目的:探讨水合淫羊藿素(icaritin)对THP-1细胞增殖和凋亡的影响及其可能的作用机制。方法:用MTS法、流式细胞术和Hoechst 33258荧光染色检测icaritin对TP-1细胞的增殖抑制及促凋亡作用,Western blot技术检测BCL-2、BAX和Caspase-3蛋白的表达。结果:4-32μmol/L icaritin作用24-72 h对THP-1细胞有增殖抑制作用(P<0.05),且呈时间-剂量依赖关系(r=0.946);4-32μmol/L icaritin作用24和48 h,对THP-1细胞有凋亡诱导作用(P<0.05),也显示时间-剂量依赖关系(r=0.924)。icaritin作用THP-1细胞48 h后,THP-1细胞BCL-2蛋白的表达较对照组显著降低(P<0.05),而BAX和Caspase-3表达较对照组显著增高(P<0.05)。结论:Icari血对THP-1细胞有增殖抑制及促凋亡作用,icaritin可能通过线粒体途径诱导THP-1细胞的凋亡。
Objective:To investigate the effect of icaritin on the proliferation and apoptosis of THP-1 cells and its mechanism.Methods:After treated with various concentrations of icaritin,cell proliferation was detected by MTS method,and apoptosis was measured with flow cytometry and Hoechst 33258 staining.Expression of BCL-2,BAX and Caspase-3 protein in THP-1 cell was detected by Western blot.Results:After treatment with various concentrations(4-32 μmol/L) of icaritin for 24,48,72 h,the inhibition rate of cell growth significantly increased(P 〈0.05) in timedose dependent manner(r = 0.946);and the apoptotic rate of cells significantly increased(P〈 0.05) in time-dose dependent manner(r = 0.924).The expression of BCL-2 protein at 48 h decreased significantly in icaritin-treated group,compared with that in control group(P 〈0.05),while the expression of BAX and Caspase3 protein at 48 h increased significantly in icaritin-treated group,compared with that in control group(P〈 0.05).Conclusion:Icaritin can inhibit proliferation and induce apoptosis of THP-1 in vitro,Icaritin may induce apoptosis in THP-1 cells through the mitochondrial pathway.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第1期94-98,共5页
Journal of Experimental Hematology
基金
国家自然科学基金(81200367)
湖南省卫计委资助项目(B2014-120)