化学发光法与酶联免疫吸附试验检测献血者梅毒螺旋体特异性抗体结果分析

Analysis of the Detection Results of the Syphilis Specific Antibody in Blood Donors by Chemiluminescence Method and Enzyme Linked Immunosorbent Assay

目的:探讨化学发光法(CMIA)检测梅毒螺旋体(TP)特异性抗体在血站血液检测中的应用价值。方法:同期采用新创酶联免疫吸附法(ELISA)和雅培化学发光法检测梅毒特异性抗体共计66 298份;对TP-ELISA法阴性而TP-CMIA法阳性无偿献血者血液样本采用免疫印迹法进行梅毒特异性抗体检测确证。结果:在66 298份无偿献血者血液样本中,TP-ELISA法检出250份阳性,阳性率0.38%;TP-CMIA法检出297份阳性,阳性率0.45%,两者差异有统计学意义(P<0.05)。结果不一致的47份无偿献血者血液样本采用TP-WB法检测确证,有32份阳性,15份阴性,而TP-ELISA法全阴性。结论:TP-CMIA法敏感性高于TP-ELISA法,具有更高的敏感性和特异性,并且检测快速,操作简便,易于自动化,在血站梅毒螺旋体检测中有较大的应用价值。

Objective： To investigate the application value of chemiluminescence method （CMIA） detection of Treponema pallidum （TP） specific antibodies in the blood test. Methods：Over the same period the de novo enzyme linked immunosorbent assay （ELISA） and Abbott chemical luminescence method were used to detect the specific antibody of syphilis in a total of 66 298 samples ; TP-ELISA negative and TP-CMIA positive unpaid blood donation blood samples for syphilis specific antibody were detected and confirmed by Western blot. Results：Blood samples from 66298 blood donors were detected by TP-ELISA, the positive samples was 250 and the positive rate was 0.38%. The positive samples of TP-CMIA was 297, the positive rate was 0.45 %, the difference was statistically significant （ P 〈 0.05 ）. The blood samples of 47 unpaid blood donors were conf＇Lrrned by TP-Western blot method, as a result, 32 samples were positive, 15 were negative, and result detected by TP-ELISA method was negative. Conclusion：TP-CMIA sensitivity is higher than that of TP-ELISA, and possesses higher sensitivity and specificity, and quick detection, simple operation, easy automation, suggesting greater application value in blood detection of Treponema pallidum.