阿帕替尼对胰腺癌细胞株AsPC-1增殖、迁移和凋亡的影响

Effect of apatinib on cell proliferation, migration and apoptosis in pancreatic cancer cell line AsPC-1

目的探讨阿帕替尼（Apatinib）对体外培养的胰腺癌AsPC-1细胞增殖、凋亡、迁移的影响。方法以不同浓度Apatinib干预胰腺癌AsPC-1细胞，采用CCK-8法和流式细胞技术检测细胞的增殖和凋亡，通过划痕实验观察Apatinib对胰腺癌细胞迁移能力的影响。结果对照组及10、20、30、40、50μmol/L Apatinib处理组AsPC-1细胞的增殖抑制率分别为0、（1.45±0.68）%、（16.92±0.70）%、（23.84±0.84）%、（34.35±1.55）%、（37.33±0.81）%，细胞增殖随Apatinib浓度的增加而显著被抑制，差异有统计学意义（P〈0.05）。对照组及20、40 μmol/L Apatinib处理组AsPC-1细胞的凋亡率分别为（9.44±0.18）%、（16.62±0.19）%、（25.42±0.41）%，细胞凋亡数量随Apatinib浓度增加而显著增多，差异具有统计学意义（P〈0.05）。对照组及20、40 μmol/L Apatinib处理组AsPC-1细胞的迁移率分别为（29.5±0.7）%、（17.4±0.9）%、（6.6±0.5）%，细胞迁移能力随Apatinib浓度增加而显著下降，差异有统计学意义（P〈0.05）。结论Apatinib能有效抑制胰腺癌AsPC-1细胞的增殖、迁移，并诱导其凋亡。

Objective To investigate the effect of apatinib on the proliferation, apoptosis and migration of pancreatic cancer cell line AsPC-1 in vitro. Methods Pancreatic cancer AsPC-1 cells were treated by apatinib in different concentrations. Cell proliferation and apoptosis were measured by CCK-8 and flow cytometry, and the effect of apatinib on cell migration ability was observed by wound healing assay. Results In control and 10, 20, 30, 40 and 50umol/L apatinib treatment group, the inhibitory rates of AsPC- 1 cells were0,（1.45±0.68）%,（16.92±0.70）%,（23.84±0.84）%,（34.35±1.55）% and （37.33± 0.81 ）% ,respectively. Cell proliferation was obviously inhibited by apatinib as the concentration increased, and the differences were statistically significant （ P 〈 0.05 ）. In control and 20, 40 umol/L apatinib treatment group, the apoptotic rates were （9.44 ± 0.18 ） %, （ 16.62 ± 0.19） % and （25.42 ± 0.41 ） %, respectively. Number of apoptotic cells was obviously increased by apatinib as the concentration increased, and the differences were statistically significant （P 〈 0.05 ）. In control and 20, 40 umol/L apatinib treatment group, the migration ability was （ 29.5 ± 0.7 ） % , （ 17.4 ± 0.9 ） % and （ 6.6 ± 0.5 ） % , which was greatly decreased as the concentration increased, and the differences were statistically significant （P 〈 0.05 ）. Conclusions Apatinib can effectively inhibit the proliferation and migration of pancreatic cancer AsPC-1 cells and induce apoptosis.