高氧环境下ROS对肠上皮细胞表达TNF-α和HIF-1-α的影响

Influence of ROS on intestinal epithelium TNF-α and HIF-1-α during hyperoxia

目的探讨长时间高氧治疗对机体造成严重不良反应的原因。方法用不同浓度的H_2O_2(100、200、400μM)和85%的高氧对肠上皮细胞干预24 h后,采用免疫组织化学方法检测高氧对TNF-α和HIF-1-α的影响。结果与对照组相比,高氧组和各H_2O_2组TNF-α和HIF-1-α表达水平显著增加(P<0.05),并且高氧组明显高于H_2O_2组。结论高氧环境中肠上皮细胞的损伤伴随着ROS的增加,因此认为在高氧环境中ROS对肠道损伤发挥着重要作用。

Objective To explore the reason of severe adverse effects by prolonged hyperoxia treatment.Methods The intestinal epithelium cells were treated with different concentrations of H2O2（100,200,400 μM） and 85% oxygen for 24 h.The expression levels of TNF-α and HIF-1-α were detected by immunohistochemistry.Results Compared with control group,the expression levels of TNF-α and HIF-1-α were significantly increased in hyperoxia group and H2O2 groups,and the expression levels of TNF-α and HIF-1-α were higher in hyperoxia group than those in H2O2 groups（P〈 0.05）.Conclusion The injury of intestinal epithelial cells in hyperoxia environment is accompanied by ROS increase.So we make a conclusion that ROS plays an important role in intestinal injury in hyperoxia environment.