白藜芦醇对间歇低氧大鼠胰岛素抵抗的影响

Effects of different doses of resveratrol on insulin resistance in rats with intermittent hypoxia

目的观察白藜芦醇对间歇低氧大鼠胰岛素抵抗的影响,探讨其可能的作用机制。方法 8周龄SD雄性大鼠40只,随机分为5组,对照组(A组)、间歇低氧组(B组)、白藜芦醇低剂量组[C组,6 mg/(kg·d)]、白藜芦醇中剂量组[D组,30 mg/(kg·d)]、白藜芦醇高剂量组[E组,60 mg/(kg·d)]。C组、D组、E组大鼠间歇低氧4周,同时按不同剂量白藜芦醇灌胃。实验结束后,测大鼠空腹胰岛素和血糖水平,取肝脏,采用RT-PCR和Western blot法测定肝脏SIRT1。结果白藜芦醇干预后间歇低氧大鼠的胰岛素抵抗降低,呈剂量依赖性,伴随SIRT1的表达增高,各组间差异有统计学意义(P<0.01)。结论白藜芦醇能减轻间歇低氧大鼠的胰岛素抵抗,呈剂量依赖性,其可能是通过提高SIRT1的表达起作用。

Objective To investigate the effects of resveratrol on insulin resistance in rats with intermittent hypoxia and to explore its underlying mechanisms.Methods Forty 8-week-old SD rats were randomly divided into five groups,control group（group A）,intermittent hypoxia group（group B）,low dose resveratrol group[group C,6 mg/（kg·d）],median dose resveratrol group[group D 30 mg/（kg·d）],high dose resveratrol group[group E,60 mg/（kg·d）].Rats in group C group D and group E were treated with different doses of resveratrol by gavage and intermittent hypoxia for 4 weeks.In the end,the fasting insulin and glucose levels of the rats were measured.The expression of SIRT1 in liver was detected by RT-PCR and Western blot.Results The insulin resistance of rats with chronic intermittent hypoxia was decreased,which was dose-dependent,and the expression of SIRT1 was increased,the difference being statistically significant（P〈 0.01）.Conclusion Resveratrol can reduce the insulin resistance in rats with intermittent hypoxia,which is dose-dependent;the mechanism may be by increasing the expression of SIRT1.