子宫内膜癌中丝裂原活化蛋白激酶4和波形蛋白的表达及临床意义

Coexpression of MAP2K4 and vimentin proteins in human endometrial carcinoma and its clinicopathological significance

目的检测子宫内膜癌中丝裂原活化蛋白激酶4(MAP2K4)和波形蛋白的表达,并分析其表达与子宫内膜癌的病理特征及预后的关系。方法采用免疫组化SP法检测128例子宫内膜癌组织中MAP2K4和波形蛋白的表达,并分析MAP2K4和波形蛋白的表达与子宫内膜癌的病理特征的相关性。结果子宫内膜癌组织中MAP2K4蛋白和波形蛋白的阳性表达率分别为49/128(38.3%)和83/128(64.8%),差异有统计学意义(P<0.001)。MAP2K4蛋白的阳性表达与肿瘤的FIGO分期及淋巴结是否转移呈负相关(P=0.010,P=0.016),而波形蛋白的阳性表达与肿瘤的FIGO分期(P=0.025)、组织学分级(P=0.017)、肌层浸润深度(P=0.044)及淋巴结是否转移(P=0.032)呈正相关。在子宫内膜癌中,MAP2K4和波形蛋白的表达呈负相关(r=-0.598,P<0.001)。MAP2K4蛋白阳性表达(MAP2K4+)的患者总体生存率较其阴性表达(MAP2K4-)的患者高(P=0.002),而波形蛋白阳性表达的患者总体生存率较其阴性表达的患者低(P=0.007)。并且,MAP2K4+/波形蛋白阴性患者的无病生存时间最长,而MAP2K4-/波形蛋白阳性患者的无病生存时间最短(P=0.004)。结论 MAP2K4和波形蛋白的联合检测有助于子宫内膜癌的早期诊断及预后分析。

Objective To analyze the expression of MAP2K4 and vimentin in human endometrial carcinoma （EC） and their association with the dinicopathological features and prognosis of the patients. Methods MAP2K4 and vimentin expressions were detected immunohistochemically in paraffin-embedded tissue sections from 128 patients with EC, and the correlation of MAP2K4 and vimentin expressions with the clinicopathological factors of the patients was analyzed. Results MAP2K4 and vimentin proteins were positively expressed in 49 （38.3%） and 83 （64.8%） of the patients, respectively. A positive expression of MAP2K4 was negatively correlated with FIGO stage of the tumor （P=0.010） and lymph node status （P=0.016）; a positive expression of vimentin was positively correlated with FIGO stage of the tumor （P=0.025）, histological grades （P=0.017）, depth of myometrial invasion （P=0.044） and lymph node status （P=0.032）. MAP2K4 was inversely associated with vimentin expression in EC（r=0.598, P〈0.001）. Patients positive for MAP2K4 tended to have a higher overall survival rate （P=0.002）, and those positive for vimentin tended to have a lower overall survival rate （P=0.007）; patients positive for MAP2K4 but negative for vimentin had the longest survival time, while those negative for MAP2K4 and positive for vimentin had lowest survival rate （P=0.004）. Conclusion Detection of MAP2K4 and vimentin might help in early diagnosis and prognostic evaluation of patients with EC.