人参皂苷Rb_1对兔耳增生性瘢痕的作用机制研究

Mechanism of Ginsenoside Rb_1 on Hypertrophic Scars of Rabbit Ears

目的:通过兔耳增生性瘢痕(HS)局部注射人参皂苷Rb_1,观察其治疗效果及探讨相关作用机制。方法:随机取8只新西兰大耳白兔,每耳6处创面,建立兔耳增生性瘢痕模型,上皮化后局部注射人参皂苷Rb_1,每3天1次,共3次。设空白组、地塞米松阳性对照组、生理盐水对照组、人参皂苷Rb_1组。注射后第1、2、4、8周切除标本行HE染色观察瘢痕增生指数(SEI),qPCR检测转化生长因子β(TGF-β)及Ⅰ型胶原表达。结果:注射后第4、8周,地塞米松及人参皂苷Rb_1组较生理盐水组HS瘢痕增生改善明显,瘢痕增生指数降低(P<0.05),TGF-β1及Ⅰ型胶原mRNA表达降低(P<0.05)。结论:人参皂苷Rb_1能有效改善兔耳增生性瘢痕,作用机制可能与抑制TGF-β1表达有关。

Objective： To observe the effect of Ginsenoside Rbl on hypertrophic scars of rabbit ears and investigate the related mechanisms. Methods ： 8 New Zealand rabbits were selected randomly and 6 wounds were made in each ear to establish the model of hypertrophic scars. After the epithelialization of the wounds, The hypertrophic scars were separated into 4 groups： Blank control group,Dexamethasone control group,Sodium chloride control group, Ginsenoside Rb1 treatment group. The injection was done once every 3 days for three times. On 1,2,4,8 weeks after the injection, The HS of 2 rabbits were harvested randomly to test HE staining and reverse transcription polymerase chain reaction（PCR）which used to test the expression of mRNA of transforming growth factor beta and Collagen I. Results： On 4 and 8 weeks after injection, HS scar improved significantly in Dexamethasone and ginsenoside Rb1 group than in the saline group,Scar elevation index decreased （P〈0.05） ,the expression of TGF-β1 and type I collagen mRNA decreased （P〈0.05）. Conclusiou： Ginsenoside Rb1 can effectively improve hypertrophic scars of rabbit ears, the mechanism may be the expression inhibition of TGF-β1 by Ginsenoside Rb1.