齿叶白鹃梅总黄酮提取物在大鼠体内药代动力学研究

Study on the Pharmacokinetic of Flavonoids Extracts of Exochorda Serratifolia Leaves in Rat Plasma

目的:研究齿叶白鹃梅总黄酮提取物在大鼠体内的药物代谢动力学特点。方法:建立大鼠血浆中黄酮类化合物在大鼠体内的代谢产物对羟基桂皮酸HPLC测定方法,考察大鼠口服齿叶白鹃梅总黄酮提取物0.083、0.167、0.333、0.500、0.667、1.0、2.0、4.0、6.0、8.0、12.0、24.0h后血药浓度变化,采用DAS 2.0数据处理软件计算药代动力学参数。结果:大鼠口服齿叶白鹃梅总黄酮提取物后,对羟基桂皮酸在大鼠体内的达峰时间T_(max)为0.618h,最大浓度C_(max)为27.881mg·L^(-1),吸收半衰期t_(1/2Ka)为0.189 h,分布半衰期t_(1/2α)为4.367h,消除半衰期t_(1/2β)为12.232h,且在4h左右时第二次达峰,出现双峰现象。结论:建立了对羟基桂皮酸血药浓度的测定方法,该方法专属性强,灵敏度高,可用于该药的体内定量分析。

Objective：To study the blood concentration of p-hydroxycinnamic after oral administration of flavonoids extracts from the leaves of Exochorda serratifolia to rats. Methods. The determination methods of p-hydroxycinnamic in leaves of Exochorda serratifolia in rat plasma was established by HPLC, the blood concentration of ρ-hydroxyeinnamic after oral administration of flavonoids extracts from leaves of Exoehorda serratifolia at 0. 083, 0. 167, 0. 333, 0.50, 0. 667, 1.0, 2.0, 4.0, 6.0, 8.0, 12.0, 24. Oh were examined, the data was dealt with DAS 2.0 data processing software to calculate the pharmacokinetics parameters. Results ： After o ral administration of flavonoids extracts from leaves of Exochorda serratifolia, the Tmax. was 0. 618h, Cmax was 27. 881mg · L1 , t1/2β, was 0. 189h, t1/2α was 4. 367h, t1/2β was 12. 232h, The second peak was appeared at 4h. Conclusion：The methods of determining the blood concentration of ρ-hydroxycinnamie was established and can be used for the analysis in vivo.