两种不同粒径二妙丸制剂药效对比研究

Comparison of the Drug Action Among Two Kinds of Grain Size of Er Miao Pill

目的:观察两种不同粒径二妙丸对佐剂性关节炎模型(AA)大鼠的改善作用及其免疫调节功能的影响。方法:将70只大鼠随机分为七组:空白组、模型组、阳性组、雷公藤多苷片组、二妙丸传统制剂与超微制剂低剂量和高剂量组,建立大鼠佐剂性关节炎模型(AA),考察二妙丸超微制剂与传统制剂对AA大鼠体重、足趾容积、脏器指数(脾脏、胸腺指数)、血清炎症因子(SOD、MDA、NO)及细胞因子IL-1β、IL-6的影响。结果:二妙丸超微制剂和传统制剂均可改善AA大鼠的足肿胀状况及炎症反应;能够升高血清中SOD含量,降低NO、MDA、IL-1β、IL-6含量;降低胸腺指数和脾脏指数;二妙丸超微制剂较其传统制剂最小有效剂量降低。结论:通过超微处理能够明显提高二妙丸的药效。

Objective：To observe the influence of two kinds of Er Miao Pill improvement and immune functions to model of adjuvant arthritis （AA） rats. Methods：Seventy rats were divided into 7 groups： blank group, model group, positive group, Er Miao Pill of traditional and ultro-micr of low-dose and high dose group, set up model of adjuvant arthritis （AA） rat, The volume of RA rats toes, organ index（spleen and thymus index）, serum inflammation factors （SOD, MDA, NO, IL-1β,IL6） were investigated to make clear the pharmacodynamic action and mechanism of ultrafine powder. Results： Both Er Miao Pill of traditional and ultro-mier can inhibit foot swelling situation of AA rats; rise SOD activity, reduced the MDA, NO, IL-1β, IL-6 content in serum, reduced thymus index and spleen index, and the effect of ultrafine powder was better than coarse powder when the dose was equal. Conclusion： It can improve drug action obviously by ultro-micr process to Er Miao Pill.