聚氧化乙烯改善大鼠慢性缺血后肢的血液灌注

Effects of polyethylene oxide on blood perfusion in the hind limbs of rats with chronic hindlimb ischemia

目的通过声学造影评价聚氧化乙烯(PEO)改善大鼠慢性缺血后肢的血液灌注的效果。方法 12只Wistar大鼠通过结扎一侧股动脉建立慢性后肢缺血模型,然后将大鼠随机分为实验组及对照组(n=6),隔天分别经颈内静脉输注PEO和生理盐水,共治疗2周。在不同的时间点行声学造影检查测定大鼠缺血后肢骨骼肌的血流量并在第28天测量血流储备量。结果从第7天开始PEO组大鼠缺血后肢骨骼肌的血流量高于对照组(P<0.05),第28天时PEO组缺血后肢骨骼肌血流储备量及血管容积均显著高于对照组(P<0.05)。结论 PEO显著提高了慢性后肢缺血模型大鼠骨骼肌的血流储备量和血流量,增加慢性缺血后肢骨骼肌的血管容积,提示PEO可能通过增加血流剪切力促进血管新生及动脉形成过程,从而改善缺血后肢的血液灌注。

Objective To evaluate the effects of polyethylene oxide （PEO） on blood perfusion in hind limb skeletal muscles in a rat model of chronic hind limb ischemia. Methods Twelve rat models of chronic hind limb ischemia established by unilateral femoral artery ligation were randomized into PEO and control groups （n=6） and treated with intravenous infusion of PEO and saline through the internal jugular vein every other day for 2 weeks. Contrast-enhanced ultrasonography was performed after the treatments to evaluate the blood flow in the skeletal muscles at different time points and blood flow reserve in the ischemic hind limbs on day 28. Results Starting from 7 days after femoral artery ligation, blood flow in the ischemic hind limb skeletal muscles was significantly higher in PEO group than in the control group （P〈0.05）. On day 28, blood flow reserve in the ischemic hind limb was significantly higher （P=0.012）, and blood volume was significantly increased in PEO group as compared that in the control group （P=0.024）. Conclusions PEO can increase blood flow, blood flow reserve and vascular volume in the hind limb skeletal muscles in rats with chronic hind limb ischemia, suggesting that PEO can promote angiogenesis and arterial formation by increasing blood flow shear stress to improve blood supply of ischemic hind limbs.