NADPH Oxidase2在胃癌组织中的表达及其与VEGF水平的相关性分析

Expression of NADPH Oxidase2 in gastric cancer and its correlation with the vascular endothelial growth factor level

目的:观察NADPH Oxidase2(NOX2)在胃癌组织中的表达及与VEGF水平的相关性。方法:2014-2015年,胃癌根治术的123例切除标本,采用免疫组织化学染色法(IHC)及Western blot检测NOX2在胃癌组织及对应的癌旁组织中的表达情况。观察癌组织中VEGF的表达情况,应用Sperman秩相关分析NOX2表达水平与VEGF水平的相关性。结果:胃癌组织中NOX2的阳性表达率为47.2%(58/123),癌旁组织中NOX2的阳性表达率为8.13%(10/123),且多为弱阳性表达(P<0.05)。NOX2胃癌组织中的表达较癌旁组织上调39.0%(48/123)。NOX2的表达与患者的性别、年龄、分化程度无关(P>0.05)与VEGF呈正相关。结论:NOX2在胃癌的发生发展中起到重要的作用,其与VEGF的表达呈正相关。

Objective： To investigate the expression of NADPH Oxidase2 （NOX2） in gastric cancer tissues and its correlation with vascular endothelial growth factor（VEGF） level. Methods： The gastric cancer tissue and the adjacent tumor tissue were obtained from the patients who received radical operation for gastric cancer during July 2014 to July 2015 in Provincial Hospital Affiliated to Shandong University. The expression of NOX2 in the tumor tissue and the adjacent tissue were detected by the immunohistochemistry（IHC） and Western blot. The VEGF level were detected by IHC in gastric cancer tissues. The spearman rank correlation were used to detected the correlation between the NOX2 and VEGF. il^s~Uz The positive expression rate of NOX2 in gastric cancer tissue was 47.2% （58/123）, and the positive expression rate in the adjacent tissue was 8.13% （10/123）, mostly expression in the adjacent was weak positive. The outcome of Western blot show that the NQX2 was up-regulated in the tumor tissue compare to the adjacent tissue [39.0% （48/123）]. The expres- sion of NOX2 and the relationship of clinical-pathology showed the expression of NOX2 had no correlation with gender, age, differentiation of tumor （X2 value were 0.852, 0.150, 5.062, P〉0.05）. The result of spearman rank correlation showed that the NQX2 was positively correlated with that of VEGF. Conclusion： NOX2 plays an important role in the genesis and development in the gastric cancer, the expression of NOX2 was closely correlated with the VEGF.