Wnt-β-catenin信号分子在急性肾损伤大鼠肾组织中的表达及意义

Wnt-β-catenin signal molecule expression level in acute kidney injury rats renal tissue

目的:探讨Wnt-β-catenin信号分子在急性肾损伤大鼠肾组织中的表达。方法:将48只大鼠按照随机数字表法分为正常对照组(Control组)、假手术组(Sham组)和缺血再灌注组(IRI组),每组16只;Control组正常喂养,IRI组建立大鼠缺血再灌注损伤模型,Sham组背侧切开后直接将伤口关闭。分别于术后第1天、第3天、第5天、第7天处死4只大鼠,观察三组大鼠肾脏组织病理学改变,并检测三组大鼠血肌酐、尿素氮水平;采用免疫印迹法检测三组大鼠肾脏组织Wnt4、β-catenin、Lrp6蛋白表达,实时定量RT-PCR检测肾脏组织Wnt4、β-catenin、Lrp6 mRNA表达。结果:Control组、Sham组各时段肾脏损伤评分、血肌酐比较差异无统计学意义(P>0.05);IRI组术后第1天至第7天肾脏损伤评分、血肌酐、尿素氮先上升后逐渐降低,其中术后第3天肾脏损伤评分、肌酐、尿素氮最高;IRI组术后1、3、5、7 d肾脏损伤评分、血肌酐、尿素氮显著高于Control组、Sham组(P<0.05)。Control组、Sham组各时段Wnt4、β-catenin、Lrp6蛋白表达差异无统计学意义(P>0.05);IRI group术后Wnt4、β-catenin、Lrp6蛋白逐渐增加,至术后第5天达表达高峰,随后逐渐降低;IRI组术后1、3、5、7 d Wnt4、β-catenin、Lrp6蛋白均显著高于Control组、Sham组(P<0.05);Control组、Sham组各时段Wnt4、β-catenin、Lrp6 mRNA表达差异无统计学意义(P>0.05);IRI group术后Wnt4、β-catenin、Lrp6 mRNA显著增加,至术后第3天达表达高峰,随后逐渐降低;IRI组术后1、3、5、7 d Wnt4、β-catenin、Lrp6 mRNA均显著高于Control组、Sham组(P<0.05)。结论:Wnt-β-catenin信号分子在急性肾损伤组织中表达显著增加,激活的Wnt-β-catenin信号通路参与了肾脏组织的修复过程。

Objective： To explore the Wnt-β-catenin signal molecule expression level in acute kidney injury rats renal tissue. Methods： A total of 48 rats accorded to the random number table method were divided into normal control group （ Control group） ,sham operation group （Sham group） and ischemia reperfusion group （IRI group） ,each group with 16 rats, Control group was given normal fed, IRI group were established ischemia reperfusion injury rats model, Sham group opened dorsal skin and then wound was closed. 4 rats were sacrificed respectively at 1,3,5,7 d after surgery, the pathological changes of kidney tissue were observed in the three groups, and the serum creatinine, urea level was detected in the three groups. Writ4, 15-catenin, Lrp6 protein expression was detected in three groups of rats by Western blot, Writ4, β-catenin, Lrp6 mRNA in renal tissues were detected by Real-time quantitative RT-PCR. Results： There was no significant difference in renal injury score and serum creatinine between Control group and Sham group （ P〉0. 05 ） , IRI group kidney injury score and serum creatinine, urea first increased and then gradually decreased, which was the highest in 3 d after surgery, IRI group renal injury score and serum creatinine, urea were significantly higher than those in Control group and Sham group 1,3,5,7 d after surgery （P〈0. 05 ）. There was no significant difference in Writ4, β-catenin, Lrp6 protein between Control group and Sham group （ P〉0. 05 ） , IRI group Writ4, β-catenin, Lrp6 protein gradually increased, which reached to peak at 5 d after surgery, and then gradually decreased, IRI group Wnt4, β-catenin, Lrp6 protein were significantly higher than those in Control group and Sham group 1,3,5,7 d after surgery（ P〈0. 05 ）. There was no significant difference in Wnt4,15-catenin, Lrp6 mRNA between Control group and Sham group （ P〉0. 05 ）, IRI group Writ4, β-catenin, Lrp6 mRNA gradually increased, which reached to peak at 3 d after surgery, and then gradually decreased, IRI group Writ4, β-catenin, Lrp6 mRNA were significantly higher than those in Control group and Sham group 1,3,5,7 d after surgery （P〈0. 05）. Conclusion： The expression of Wnt-β-catenin signal molecule are significantly increase in acute kidney injury, and the activation of Wnt-β-catenin signal pathway is involved in the repair process of renal tissue.