异源表达大豆GmNRT1-like提高拟南芥对低氮的耐受性

Heterologous Expression of NRT1-like From Soybean Confers Tolerance to Nitrogen Deficiency in Arabidopsis

氮素是植物生长发育所需的重要元素,提高作物对氮素的利用效率可以减少化肥施用量并减轻对环境的污染,因此挖掘新的氮高效(NUE)基因成为了分子育种中的一项重要任务.在大豆中克隆到一个低氮上调表达的硝酸盐运输载体(NRT)家族成员Gm NRT1-like,进行了双元表达载体的构建并转化模式植物拟南芥,并在转基因拟南芥中对该基因功能进行了研究.研究结果表明,在低氮胁迫条件下,转基因植株产生更多的侧根,即其根系更发达.证明异源表达大豆Gm NRT1-like基因提高了拟南芥对低氮的耐受性.

Nitrogen is an essential element for plant growth and development. Improving Nitrogen Use Efficiency （NUE） of crops could potentially reduce the application of chemical fertilizer and alleviate environ- mental damage. To identify new NUE genes is therefore an important task in molecular breeding. In the present study, a soybean nitrate transporter （NRT） member, GmNRTl-like, was analyzed under nitrogen deficient condition. The transcript level of GmNTRl-like significantly increased under N deficient condition. Thereafter, the GrnNRTl-like was overexpressed in Arabidopsis for gene functional analysis. The transgen- ic lines produced more lateral roots than wild type under nitrogen deficient condition. Our findings suggest- ed that heterologous expression of NRTl-like from soybean might confer nitrogen deficiency tolerance in Arabidopsis.