苹果树腐烂病病原菌培养条件研究

Study on Culture Conditions of Apple Tree Canker Pathogen

在不同培养条件下对苹果树腐烂病病原菌生长情况进行了研究。结果表明,在试验改造条件下苹果树腐烂病病原菌生长最好的培养基为PDA,其余依次为苹果树皮、苹果果汁、玉米粉培养基,在查比克培养基上生长最差。病原菌可利用多种碳源和氮源,以麦芽糖或淀粉为碳源时生长好于葡萄糖,在几种不同碳源培养基上生长差异不显著。光照对病原菌的生长也有一定的影响,前期是12 h光照交替培养生长最快,全光照次之,到96 h时两者差异不显著,但均显著好于全黑暗。生长的最适p H为4和5,随p H增大生长变差,p H≥9时不能生长。

The growth of apple tree canker bacteria had been studied under different culture conditions , the result shows that the apple tree canker pathogen the best medium for growth is PDA, followed by the bark, fruit juice, corn meal medium, the growth on Czapek medium is the worst under the test condition; the bacteria can make use of a variety of carbon and nitrogen source, it grew better when took maltose or starch as carbon source than glucose, the growth on different carbon sources medium had no significant difference; grew optimum at pH is 4 or 5, deterioration growth along with the increasing pH, it can not grow when pH≥9; at the same time light effected the growth of pathogenic bacteria appreciably, under 12 hours alternation condition it grew fastest followed by full light, 96 hours after there is no significant difference between the two treatment, but they both grew significantly better than the whole darkness treatment.