不同来源精子对体外受精患者胚胎和妊娠结局的影响

Effect of Spermatozoa from Different Sources on Embryos and Pregnancy Outcomes of External Fertilization

目的探讨不同来源精子对体外受精患者胚胎和妊娠结局的影响。方法回顾性分行卵胞浆内单精子注射(ICSI)技术治疗的患者21例,依据精子来源分为三组各7例。A组患者给予手淫法取精,B组患者给予睾丸穿刺取精术取精,C组给予经皮附睾穿刺取精术取精。结果在受精成功率、卵裂率、优质胚胎率方面,C组明显高于A组、B组(P<0.05),但A组与B组无统计学差异(P>0.05)。三组患者的妊娠率、胚胎种植率均无统计学差异(P>0.05)。结论经皮附睾穿刺取精术可有效提高患者的体外受精成功率,且其胚胎发育潜能良好,但对妊娠结局无显著影响。

Objective To discuss the effect of spermatozoa from different sources on embryos and pregnancy outcomes of external fertilization. Methods 21 cases of patients with 1CSI technology were analyzed retrospectively. According to the sources of spermatozoa, all cases were divided into ejaculated spermatozoa group （group A）, testicular sperm aspiration （group B）, and percutaneous epididymal sperm aspiration group （group C）, with 7 cases in each group. Results In fertilization success rate, cleavage rate, high quality embryo rate, group C was significantly higher than group A and group B （P〈0.05）, while no statistical difference was found between group A and group B （P〉0.05）. No statistical difference was found in the pregnancy rate and embryo implantation rate among 3 groups （P〉0.05）. Conclusions Percutaneous epididymal sperm aspiration can effectively improve the success rate of external fertilization, and the potential of embryonic development is good, but there is no significant effect on pregnancy outcome.