转CP4-EPSPs基因大豆杂交后代对草甘膦的抗性水平与遗传背景的相关性

Correlation between Resistance to Glyphosate and Genetic Background in Transgenic CP4-EPSPs Gene Soybean Progeny

第一代抗草甘膦转CP4-EPSPs基因大豆GTS40-3-2是国际上应用时间最长、种植面积最大的转基因作物。本文以6份GTS40-3-2衍生的抗草甘膦转基因大豆新品系为亲本,配制4个杂交组合,利用抗性分级法和相对株高法鉴定杂交亲本及其F_(2:3)子代对草甘膦的耐受性差异,分析其抗性水平与遗传背景的相关性。结果表明,以1230 g a.i.hm^(–2)草甘膦喷施处理时,转基因亲本及其F_(2:3)子代的苗期生长受草甘膦抑制不显著,而当喷施浓度提高至3690 g a.i.hm^(–2)和4920 g a.i.hm^(–2)时则抑制作用显著。供试的6个杂交亲本中以ZLHJ06-1568、ZLHJ10-713和ZLHJ06-698对草甘膦的耐受性相对较强,而4个F_(2:3)组合中以ZLHJ10-713×ZLHJ06-698后代在草甘膦喷施后株高受抑制最小,对草甘膦耐受性最强。不同组合后代对草甘膦的耐受性普遍优于其双亲,呈现出杂种优势。各组合后代与亲本之间对草甘膦的耐受性均呈正相关,但由于亲本间互作效应的不同,导致后代抗性水平产生差异。本研究表明草甘膦抗性基因CP4-EPSPs在大豆中的表达水平与其遗传背景相关联,为利用转基因大豆新种质培育转基因大豆新品种过程中目标基因的定向选择提供了参考依据。

The first Roundup Ready（RR） soybean（GTS40-3-2） carrying CP4-EPSPs is one of the transgenic crops that have the longest application time and largest planting acreage worldwide.However,it has not been commercialized and has less report in China.In this study,six RR soybean lines derived from GTS40-3-2 were hybridized to create four combinations.We used resistance grading and relative plant height in identifying the resistance to glyphosate of parents and F_（2：3） progeny,to investigate the correlation between resistance of the progeny and genetic background.The growth inhibition of transgenic parents and progeny at seeding stage was not significant when the glyphosate treatment concentration was 1230 g a.i.hm^（–2）,while it was significant when the glyphosate concentration was 3690 g a.i.hm^（–2） and 4920 g a.i.hm^（–2）.ZLHJ06-1568,ZLHJ10-713,and ZLHJ06-698 among the six parents had the more glyphosate resistance relatively.The progeny of ZLHJ10-713×ZLHJ06-698 combination had the least inhibition of plant height showing highest resistance to glyphosate in all four F_（2：3） combinations.The progeny had better resistance to glyphosate than their parents showing better heterosis.The glyphosate resistance of progeny was positively correlated with that of parents.Difference of glyphosate resistance was found in progeny among different combinations because of the interaction between parents.Our study indicates that the expression of glyphosate resistance gene is closely related to the genetic background in soybean,providing a theoretical evidence for target gene selection in developing new varieties by using transgenic germplasm.