固定化黄孢原毛平革菌对栲胶降解的研究

Effect of immoblized phanerochaete chrysosporium on vegetable tannin extract degradation

将白腐菌黄孢原毛平革菌包埋于海藻酸钙中,制备了固定化黄孢原毛平革菌凝胶颗粒,并研究栲胶溶液及栲胶铬共基质条件下,固定化黄孢原毛平革菌对栲胶的降解影响。运用Box-Behnken Design(BBD)研究影响栲胶降解的因素。试验结果表明:当栲胶浓度为400mg/L,处理时间为10d时,固定化黄孢原毛平革菌对栗木的降解率为89.81%、荆树皮降解率为91.03%、槟榔降解率为76.53%。在铬共基质条件下固定化的黄孢原毛平革菌对栗木的降解率可以达到94.40%、荆树皮的降解率为92.43%、槟榔的降解率为83.11%。

The immobilized phanerochaete chrysosporium gel particles were prepared by making the white rot fungus phaneroehaete chrysosporium embed in Ca-alginate. The effect of immobilized phanerochaete chrysosporium on degra- dation rate of vegetable tannin extract under the vegetable tannin extract solution and chromium co-substrates condi- tions was researched. The influencing factors of vegetable tannin extract degradation were studied by Box - Behnken Design （BBD）. Results show that under the condition of 400mg/L vegetable tannin extract concentration and 10 days treatment, the degradation rates of immobilization phanerochaete chrysosporium to the chestnut extract, mimosa extract and gambier extract are 89. 81%, 91.03%, and 76. 53% respectively. In chromium co-substrates solution the degradation rates of immobilized phanerochaete chrysosporium to the chestnut extract, mimosa extract and gambier extract can reach to 94.40%, 92.43% and 83.11% respectively.