玉米瘤黑粉病与丝黑穗病高效接种方法的筛选

Screening of Efficient Inoculation Methods of Head Smut and Common Smut of Corn

本研究以高感品种吉单209为材料,接种玉米黑粉菌与玉米丝黑穗菌,采用玉米茎基部注射、拌菌土、玉米苗期灌根、菌液浸种等4种方法,在模拟田间环境条件下观察玉米植株的发病情况,并对玉米植株发病情况进行对比分析,筛选适合玉米黑粉病与玉米丝黑穗病的接种方法。结果显示,玉米茎基部注射是玉米瘤黑粉病最佳接种方法。玉米茎基部注射和菌液浸种是玉米丝黑穗病较好的两种接种方法。本研究为今后快速评价玉米资源对玉米瘤黑粉病及玉米丝黑穗病抗性提供可靠的接种方法。

Jidan209, a corn variety highly susceptible to head smut and common smut of corn, was selected as mate-rial and inoculated with Ustilago maydis and Sporisorium reilianum. Four methods were adopted, including injec-tion of corn coleoptile, mixing soil with the disease fungus, watering the roots of maize seedlings with liquid culturesof Ustilago maydis and Sporisorium reilianum, soaking maize seeds with liquid cultures of Ustilago maydis and Sporisorium reilianum. Screening The efficient inoculation methods of head smut and common smut of corn werescreened by comparing and observing the incidence of corn plants under the simulation of the field condition. Theresults showed that injection of corn coleoptile was the best inoculation method for common smut of corn. Injectionof corn coleoptile and soaking maize seeds with culture liquids of Sporisorium reilianum were better than other inoc-ulation methods of head smut of corn. This study will be beneficial for us to screen cron varieties resistant to headsmut and common smut of corn.