沙柳PNY基因克隆、生物信息学及组织特异性表达

PNY Gene Cloning,Bioinformatics and Tissue-specific Expression Analysis of Salix psammophila

为了促进沙柳(Salix psammophila)分子定向育种,研究了其分枝及材性发育相关遗传机制。克隆获得了沙柳PNY基因。研究发现:沙柳PNY基因编码区全长1 446 bp,编码481个氨基酸残基。PNY蛋白由55.09%无规卷曲、29.73%α-螺旋及15.18%延伸链构成。保守结构域分析表明,该蛋白分子存在PNY蛋白典型的SKY、BELL、Homeodomain结构域。系统进化分析表明,沙柳与杞柳(Salix purpurea)、毛果杨(Populus trichocarpa)的PNY基因亲缘关系最近。qRT-PCR组织特异性表达分析发现,PNY基因在沙柳腋芽部位表达量最高,茎、叶、顶芽、根中表达量依次降低。

To promote the molecular breeding of ＂Salix psammophila, we studied its branching and wood formation mechanism. PNY gene was cloned from S. psammophila. PNY gene has 1 446 bp coding sequences which encodes a polypeptide of 481 amino acids, and this protein is consist of the 55.09% random coil, 29.73% or-helix and 15.18% extended strand. By the conserved domains analysis, there are conserved SKY, BELL and Homeodomain in PNY protein. By the phylogenetic analysis, PNY of S. psammophila has closer relationship with those of S. purpurea and P. trichocarpa. The results of qRT-PCR in different tissues of S. psammophila show that the highest level of the PNY expression is in the leaf axil, and the expression levels are decreased successively in stem, leaves, apical buds and roots.