响应曲面法优化猴头菇粗多糖的提取工艺

Optimization of Extraction Process for Crude Polysaccharide from Hericium Erinaceus by Response Surface Methodology

应用Box-Behnken设计-响应面法优化猴头菇粗多糖的提取工艺。以提取时间、液料比、温度为影响粗多糖提取率的主要考察因素,通过Box-Behnken试验设计,以猴头菇粗多糖提取率为响应值,应用Designexpert 7.0软件对结果进行二次多项式模型拟合,绘制等高线图和响应面图,比较各因素交互作用对提取率影响的强弱,得出最优工艺,并对其进行验证。结果显示最佳工艺条件为:提取时间3.8 h,液料比25 m L/g,提取温度81℃。验证实验显示猴头菇粗多糖提取率为3.09%,实测值与预测值接近。研究结果说明响应曲面法建立的模型预测性良好,能合理地优化猴头菇中粗多糖的提取工艺。

Applying Box-Behnken design and response surface methodology,this study optimized the extraction conditions for crude polysaccharide from Hericium erinaceus investigation.The extraction time,ratio of water to raw material and extraction temperature were selected as the key investigation factors.Box-Behnken design took the extraction rate as the response value.Design Expert software 7.0 was used to establish quadratic multinomial models fitting for experimental data.Contour overlay plot and response surface were delineated for the comparison of interactions of each key factor.And then the optimal formulation was screened out and validated.The result showed that the optimal extraction parameters were as follows：extraction time 3.8 h,ratio of water to raw material 25 mL/g,extraction temperature 81℃.The results of validation test showed that the extraction rate was 3.09%,basically met with the forecast value.Research results showed the model established by the response surface methodology had good predictability,and could rationally optimize the extracting technology for crude polysaccharide from Hericium erinaceus.